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Transepithelial Transport of the Bioactive Tripeptide, Val-Pro-Pro, in Human Intestinal Caco-2 Cell Monolayers
Open accessAbstract
Some of the food-derived tripeptides with angiotensin converting enzyme (ACE)-inhibitory activity have been reported to be hypotensive after being orally administered. The mechanism for the intestinal transport of these tripeptides was studied by using monolayer-cultured human intestinal Caco-2 cells which express many enterocyte-like functions including the peptide transporter (PepT1)-mediated transport system. Val-Pro-Pro, an ACE-inhibitory peptide from fermented milk, was used as a model tripeptide. A significant amount of intact Val-Pro-Pro was transported across the Caco-2 cell monolayer. This transport was hardly inhibited by a competitive substrate for PepT1. Since no intact Val-Pro-Pro was detected in the cells, Val-Pro-Pro apically taken by Caco-2 cells via PepT1 was likely to have been quickly hydrolyzed by intracellular peptidases, producing free Val and Pro. These findings suggest that PepT1-mediated transport was not involved in the transepithelial transport of intact Val-Pro-Pro. Paracellular diffusion is suggested to have been the main mechanism for the transport of intact Val-Pro-Pro across the Caco-2 cell monolayer.
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Citations
Cited by
Food-Derived Bioactive Peptides in Human Health: Challenges and Opportunities
Chakrabarti, S., Guha, S., & Majumder, K. (2018) · Nutrients · doi:10.3390/nu10111738 · Cited by 699
Bioactive peptides and proteins from foods: indication for health effects.
Möller, N. P., Scholz-Ahrens, K. E., Roos, N., & Schrezenmeir, J. (2008) · European Journal of Nutrition · doi:10.1007/s00394-008-0710-2 · Cited by 595
Bioactive peptides and lactic fermentations.
FITZGERALD, R. J., & MURRAY, B. A. (2006) · International Journal of Dairy Technology · doi:10.1111/j.1471-0307.2006.00250.x · Cited by 355
De Leo, F., Panarese, S., Gallerani, R., & Ceci, L. (2009) · Current Pharmaceutical Design · doi:10.2174/138161209789271834 · Cited by 149
Effects of Lactobacillus helveticus fermented milk on bone cells in vitro
Narva, M., Halleen, J., Väänänen, K., & Korpela, R. (2004) · Life Sciences · doi:10.1016/j.lfs.2004.04.011 · Cited by 87
Metadata
- Authors
- SATAKE, M., ENJOH, M., NAKAMURA, Y., TAKANO, T., KAWAMURA, Y., ARAI, S., & SHIMIZU, M.
- Venue
- Bioscience, Biotechnology, and Biochemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-01
- Volume
- 66
- Issue
- 2
- Pages
- 378-384
- ISSN
- 0916-8451 (ISSN-L)
- 1347-6947
- OpenAlex
- W1984153473
- Cited by
- 237
- References
- 0
- FWCI
- 1.94
- Type
- journal article
- Language
- en
- Open access
- bronze
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- SATAKE, M., ENJOH, M., NAKAMURA, Y., TAKANO, T., KAWAMURA, Y., ARAI, S., & SHIMIZU, M. (2002). Transepithelial Transport of the Bioactive Tripeptide, Val-Pro-Pro, in Human Intestinal Caco-2 Cell Monolayers. Bioscience, Biotechnology, and Biochemistry. https://doi.org/10.1271/bbb.66.378
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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