The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Disruption of rpmJ Encoding Ribosomal Protein L36 Decreases the Expression of secY Upstream of the spc Operon and Inhibits Protein Translocation in Escherichia coli
Abstract
The spc operon of Escherichia coli encodes 11 ribosomal proteins and SecY. The secY gene and downstream rpmJ encoding a ribosomal protein, L36, are located distal to the promoter of the spc operon. It has been suggested that the stability of SecY mRNA depends on rpmJ unless a rho-independent terminator is inserted immediately downstream of secY. Moreover, it has been suggested that RpmJ is dispensable for E. coli. We constructed rpmJ null strains, AY101 (DeltarpmJ::tetA) and AY201 (DeltarpmJ::cat), by replacing rpmJ with tetA, which encodes a membrane protein responsible for tetracycline-resistance, and cat, which encodes a cytoplasmic chloramphenicol acetyltransferase, respectively. Depletion of RpmJ did not inhibit protein synthesis, whereas the growth of AY101 was defective at high temperatures. The level of SecY mRNA decreased significantly in both disruptants even though the rho-independent terminator was inserted immediately downstream of secY. Some periplasmic proteins were missing in the disruptants with a concomitant increase in the amount of phage shock protein in the inner membrane. These phenotypes caused by the rpmJ null mutation were corrected by a plasmid carrying secY, but not by one carrying rpmJ.
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“These genes are significantly down-regulated during stationary phase or when cells encounter stress conditions (Coenye and Vandamme, 2005; Ikegami et al., 2005; Starosta et al., 2014).”
From The Dynamic SecYEG Translocon (2021) · cites this paper as Ikegami et al., 2005
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“This is rather surprising, because secY is encoded in the spc operon together with genes for several ribosomal proteins (Lindahl et al., 1990; Ikegami et al., 2005).”
From The Dynamic SecYEG Translocon (2021) · cites this paper as Ikegami et al., 2005
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Cites
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Use of Bacteriophage λ Recombination Functions To Promote Gene Replacement in Escherichia coli
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[41] Reconstitution of ribosomes from subribosomal components
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Oswald, J., Njenga, R., Natriashvili, A., Sarmah, P., & Koch, H.-G. (2021) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2021.664241 · Cited by 88
Metadata
- Authors
- IKEGAMI, A., NISHIYAMA, K.-I., MATSUYAMA, S.-I., & TOKUDA, H.
- Affiliations
- Rikkyo University (JP)
- Institute for Quantitative Biosciences, The University of Tokyo (JP)
- Venue
- Bioscience, Biotechnology, and Biochemistry
- Publisher
- Informa UK Limited
- Published
- 2005-01
- Volume
- 69
- Issue
- 8
- Pages
- 1595-1602
- ISSN
- 0916-8451 (ISSN-L)
- 1347-6947
- OpenAlex
- W2008802977
- Cited by
- 30
- References
- 34
- Citations by year
- 2026: 6 · 2024: 1 · 2023: 3 · 2022: 1 · 2021: 2 · 2020: 2 · 2019: 2 · 2018: 1 · 2015: 1 · 2014: 3 · 2013: 1 · 2012: 1
- FWCI
- 0.72
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioscience Biotechnology and Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
- Keywords
- SecY, Escherichia coli, mRNA stability, rho-independent terminator, protein translocation, periplasmic proteins, phage shock protein, gene expression regulation
- MeSH terms
- SEC Translocation Channels, Base Sequence, Genes, Bacterial, Molecular Sequence Data, Operon, Ribosomal Proteins, DNA Primers, Protein Transport, Escherichia coli Proteins
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