Cited by 148Tea Polyphenols and EffectsFood and Agricultural SciencesHeavy Metals in Plants

Studies on the Chemical Constituents of Tea

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SAKATO, Yajiro·1950·Journal of the agricultural chemical society of Japan

Abstract

The author discovered a new amide in the water extract of Japanese green tea, and named it “theanine”. This substance crystallizes in the form of colorless needle; molecular formula C7H14O3N2, melting point 217_??_218° (decompose), and [α]12 D=+7.1. It presents strong ninhydrin reaction. It is very soluble in water, and is dissolved in 2.6 times of water at 0° and 1.8 times of water at 100°, but insoluble in ethyl alcohol and ether. By hydrolysis of theanine, it gives L-glutamic acid and ethyl amine nearly quantitatively. From the fact that it shows no biuret reaction and that the natural glutamine is γ-amide, it seems very probable that theanine may be L-glutamic acid γ-ethyl amide. It is the first time that ethyl amine has been discovered, though not in a free state, in plant kingdom. The author has also studied the distribution of theanine in each stage of tea leaf developments. Gyokuro is rich (about 1%) in it, but Sencha is poor. It seems, therefore, that theanine assumes the analogous rôle of glutamine or asparagine in other plants though the destination of ethyl amine is yet unknown.

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Crossref · 10.1271/nogeikagaku1924.23.262

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Authors
SAKATO, Y.
Venue
Journal of the agricultural chemical society of Japan
Published
1950
Volume
23
Issue
6
Pages
262-267
ISSN
  • 0002-1407 (ISSN-L)
  • 1883-6844
OpenAlex
W2323839409
Cited by
148
References
0
Citations by year
2026: 2 · 2025: 9 · 2024: 4 · 2023: 3 · 2022: 6 · 2021: 11 · 2020: 3 · 2019: 11 · 2018: 5 · 2017: 3 · 2016: 3 · 2015: 4 · 2014: 7 · 2013: 6 · 2012: 8
FWCI
0.00
Type
journal article
Language
ja
Open access
Locations
Nippon Nōgeikagaku Kaishi (publishedVersion)
Topics
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Tea Polyphenols and Effects
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
  • Physical Sciences › Chemistry › Analytical Chemistry › Heavy Metals in Plants
Keywords
theanine, green tea, Japanese green tea, amides, glutamate, ethylamine, glutamine, asparagine, sencha, ninhydrin reaction, hydrolysis

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