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Quantitative determination of bovine serum haptoglobin and its elevation in some inflammatory diseases.
Open accessAbstract
A modification of the method of Tarukoski was employed for the determination of bovine serum haptoglobin (Hp). A mixture of serum sample and diluted methemoglobin (Hb+) solution (30 mg/100 ml) was incubated with o-dianisidine reagent, pH 4.1, at 37°C for 45 min. Peroxidase activity of free Hb was found almost lost in the mixture, in which HpHb complex activity was well maintained. Relatively reliable values were obtained even in hemolyzed serum samples as long as the contaminating Hb was less than 150 mg/100 ml. The Hp values determined by this method were quite parallel to the results obtained by polyacrylamide gel disc electrophoresis. They were small or none in normal cattle, but remarkably large (80-130mg% HbBC) in cattle suffering from severe inflammatory diseases, such as mastitis, pyometra and traumatic reticulitis.
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Cited by
Acute phase proteins in dogs and cats: current knowledge and future perspectives
Cerón, Eckersall, & Martínez‐Subiela, S. (2005) · Veterinary Clinical Pathology · doi:10.1111/j.1939-165x.2005.tb00019.x · Cited by 829
Bovine and canine acute phase proteins
Eckersall, P. D., & Conner, J. G. (1988) · Veterinary Research Communications · doi:10.1007/bf00362798 · Cited by 273
Arthington, J. D., Eicher, S. D., Kunkle, W. E., & Martin, F. G. (2003) · Journal of Animal Science · doi:10.2527/2003.8151120x · Cited by 259
Heegaard, P. M. H., Godson, D. L., Toussaint, M. J. M., Tjørnehøj, K., Larsen, L. E., Viuff, B., & Rønsholt, L. (2000) · Veterinary Immunology and Immunopathology · doi:10.1016/s0165-2427(00)00226-9 · Cited by 249
Serum haptoglobin as an indicator of the acute phase response in bovine respiratory disease
Godson, D. L., Campos, M., Attah-Poku, S. K., Redmond, M. J., Cordeiro, D. M., Sethi, M. S., Harland, R. J., & Babiuk, L. A. (1996) · Veterinary Immunology and Immunopathology · doi:10.1016/0165-2427(95)05520-7 · Cited by 201
Metadata
- Authors
- MAKIMURA, S., & SUZUKI, N.
- Venue
- The Japanese Journal of Veterinary Science
- Published
- 1982
- Volume
- 44
- Issue
- 1
- Pages
- 15-21
- ISSN
- 0021-5295 (ISSN-L)
- 1881-1442
- OpenAlex
- W2052946693
- Cited by
- 324
- References
- 0
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- bronze
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- MAKIMURA, S., & SUZUKI, N. (1982). Quantitative determination of bovine serum haptoglobin and its elevation in some inflammatory diseases. The Japanese Journal of Veterinary Science. https://doi.org/10.1292/jvms1939.44.15
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