Morphological analysis of patchy thickening and reddish discoloration of active hair growth areas in the skin of New Zealand White rabbits
Open accessAbstract
Patchy thickening and reddish discoloration of active hair growth areas of skin in rabbits are occasionally found, and this gross feature could affect precise evaluation when conducting a dermal irritation test. Since little is known about the mechanism of this phenomenon, we examined the dorsal skin of New Zealand White rabbits morphologically and immunohistochemically in order to identify the possible mechanism responsible for developing these skin changes in relation to the hair cycle. Skin samples from 4 rabbits were divided into three groups (5 samples/group) based on their macroscopic characteristics: a thickened skin, erythematous skin, and smooth skin group. Histomorphological examination revealed that the percentage of hair follicles in the anagen phase, hair follicle length, hair follicle area, and proliferating cell nuclear antigen-positive cells in the hair follicles were greater in the thickened skin and erythematous skin groups than in the smooth skin group. Unlike mice and rats, the dermis was nearly adjacent to the muscular layer with a thin hypodermis, and the whole lengths of hair follicles in the anagen phase were located in the dermis in the rabbit skin. These results suggest that large hair follicles in the anagen phase compressed the surrounding dermis; therefore, the skin was grossly raised and showed thickening. A higher number of CD31-positive blood vessels, suggesting the occurrence of angiogenesis, was observed around the hair follicles in the erythematous skin group, and they seemed to affect the reddish discoloration of skin noted grossly.
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Irregular hair spots in rabbits have been reported to appear as raised, thickened islands compared to the surrounding skin.
“Irregular hair spots in rabbits have been reported to appear as raised, thickened islands compared to the surrounding skin38.”
From Morphological characteristics and notes of the skin in preclinical toxicity assessment (2023) · cites this paper as [38]
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Morphological characteristics and notes of the skin in preclinical toxicity assessment
Moroki, T. (2023) · Journal of Toxicologic Pathology · doi:10.1293/tox.2022-0103 · Cited by 26 · see citing works
- Authors
- Ishihara, T., Yamashita, H., Sakurai, T., Morita, J., Sakamoto, K., Ishii, A., & Sasaki, M.
- Affiliations
- Taisho Pharmaceutical (Japan) (JP)
- Corresponding author
- Ishihara, Tomoko
- ORCID
- Sasaki, Minoru
- Venue
- Journal of Toxicologic Pathology
- Published
- 2017
- Volume
- 30
- Issue
- 4
- Pages
- 315-322
- ISSN
- 0914-9198 (ISSN-L)
- 1881-915X
- PMC
- PMC5660953
- OpenAlex
- W2746534894
- Cited by
- 8
- References
- 0
- Citations by year
- 2024: 3 · 2022: 1 · 2020: 2 · 2019: 2
- FWCI
- 1.53
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Journal of Toxicologic Pathology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Health Sciences › Medicine › Dermatology › Contact Dermatitis and Allergies
- Life Sciences › Immunology and Microbiology › Immunology › Immunotoxicology and immune responses
- Keywords
- hair cycle, anagen phase, hair follicle, dermis, angiogenesis, proliferating cell nuclear antigen, rabbit skin, New Zealand White rabbits, skin morphology
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