Sensitivity and specificity of ANA and anti-dsDNA in the diagnosis of systemic lupus erythematosus: A comparison using control sera obtained from healthy individuals and patients with multiple medical problems
Abstract
Background Antinuclear antibodies (ANA) and anti-double stranded DNA (anti-dsDNA) are often tested as a screening tool in patients with suspected systemic lupus erythematosus or connective tissue diseases. ANA can be seen in healthy controls (HC) and patients with multiple medical problems (MMP). Objective To determine the sensitivity and specificity of ANA and anti-dsDNA in SLE patients, using sera from HC and MMP patients. Methods Serum samples from HC, MMP and SLE patients, 100 in each group, were analyzed for the presence of ANA and anti-dsDNA, by indirect immunofluorescent assay, using a HEp-2 cell and Crithidia luciliae as substrates, respectively. Results The prevalence of ANA at a titer of ≥1:80 and ≥ 1:160 was 8% and 4%, respectively, in HC; and it was 12% and 6% respectively, in MMP patients. The prevalence of anti-dsDNA was 0% in HC and 3% in MMP patients. When using HC sera for the diagnosis of SLE, the sensitivity of ANA at a titer of ≥ 1:80 and ≥ 1:160 was 98% and 90%, respectively, with specificity of 92% and 96%, respectively. The specificity decreased to 88% and 94%, respectively, when using sera from MMP patients. The specificity of anti-dsDNA was 100% and 97%, when using sera from HC and MMP patients, respectively. Conclusion ANA and anti-dsDNA gave high sensitivity and high specificity in patients with SLE, even when using MMP patient's sera as controls. Physicians should take care in interpreting ANA and anti-dsDNA results in MMP patients who do not have…
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ELISA is a useful and widely used laboratory test to screen for anti-dsDNA Abs because of its high sensitivity, quantitative results, simplicity, and relatively low cost.
“ELISA is a useful and widely used laboratory test to screen for anti-dsDNA Abs [13,18] because of its high sensitivity, quantitative results, and simplicity, as well as relatively low cost compared to other assays [19,20]”
From Anti-double stranded DNA antibodies: A rational diagnostic approach in limited-resource settings (2022) · cites this paper as [18]
Cites
Antico, A., Platzgummer, S., Bassetti, D., Bizzaro, N., Tozzoli, R., Villalta, D., & Study Group on Autoimmune Diseases of the Italian Society of Laboratory Medicine (SIMeL) (2010) · Lupus · doi:10.1177/0961203310362995 · Cited by 70 · see citing works
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Leuchten, N., Hoyer, A., Brinks, R., Schoels, M., Schneider, M., Smolen, J., Johnson, S. R., Daikh, D., Dörner, T., Aringer, M., et al. (2018) · Arthritis Care & Research · doi:10.1002/acr.23292 · Cited by 190 · see citing works
Schmajuk, G., Hoyer, B. F., Aringer, M., Johnson, S. R., Daikh, D. I., Dörner, T., & the SLE classification criteria steering committee and the international SLE expert panel of the initiative (2018) · Arthritis Care & Research · doi:10.1002/acr.23503 · Cited by 65 · see citing works
Anti-double stranded DNA antibodies: A rational diagnostic approach in limited-resource settings
Admou, B., Eddehbi, F.-E., Elmoumou, L., Elmojadili, S., Salami, A., Oujidi, M., Brahim, I., & Hazime, R. (2022) · Practical Laboratory Medicine · doi:10.1016/j.plabm.2022.e00285 · Cited by 8 · see citing works
“ELISA is a useful and widely used laboratory test to screen for anti-dsDNA Abs [13,18] because of its high sensitivity, quantitative results, and simplicity, as well as relatively low cost compared to other assays [19,20], while CLIFT provides higher clinical specificity than ELISA [10].”
Result
- Authors
- Wichainun, R.
- Affiliations
- Chiang Mai University (TH)
- Corresponding author
- Wichainun, Ramjai
- Venue
- Asian Pacific Journal of Allergy and Immunology
- Published
- 2013-04-29
- Volume
- 31
- Issue
- 4
- Pages
- 292-8
- ISSN
- 0125-877X (ISSN-L)
- OpenAlex
- W2090687019
- Cited by
- 82
- References
- 1
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 8 · 2023: 7 · 2022: 10 · 2021: 12 · 2020: 4 · 2019: 9 · 2018: 6 · 2017: 4 · 2016: 8 · 2015: 5 · 2014: 3 · 2013: 1
- FWCI
- 4.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Asian Pacific Journal of Allergy and Immunology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Rheumatology › Systemic Lupus Erythematosus Research
- Health Sciences › Medicine › Pathology and Forensic Medicine › Systemic Sclerosis and Related Diseases
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Vasculitis and related conditions
- Keywords
- systemic lupus erythematosus, antinuclear antibodies, anti-dsDNA antibodies, sensitivity and specificity, healthy controls, connective tissue diseases, indirect immunofluorescence, HEp-2 cells, Crithidia luciliae, autoantibody detection
- MeSH terms
- Adult, Antibodies, Antinuclear, Autoantibodies, Autoantigens, DNA, Female, Humans, Lupus Erythematosus, Systemic, Male, Middle Aged, Sensitivity and Specificity, Fluorescent Antibody Technique, Indirect
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