The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Euthynnus affinis viscera-an alternative source for protease and lipase enzymes: Characteristic and potential application as destainer agent: Euthynnus affinis viscera as source for protease and lipase enzyme
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Abstract. Mardina V, Harmawan T, Fitriani, Sufriadi E, Febriani, Yusof F. 2020. Euthynnus affinis viscera-an alternative source for protease and lipase enzymes: Characteristic and potential application as destainer agent. Biodiversitas 21: 5858-5864. This study described the characterization of protease and lipase, extracted from the viscera of Euthynnus affinis and their applications as stains removal. Enzyme increased a few folds (3.99 folds for protease and 2.45 folds for lipase) after the submission to partial purification processes by ammonium sulfate precipitation and dialysis. Molecular mass assessment by SDS-PAGE showed that the enzyme solution contains three protein bands, estimated at 70, 42, and 24 kDa for protease and 32 kDa for lipase. Zymography confirmed the presence of protease and lipase in the sample. The protease and lipase exhibited optimum activities at pH 7.0 and pH 8.0 respectively, active at temperatures from 35 to 75oC with optimal activities at 65oC for both. The enzymes were stable at alkaline pH after 90 minutes (mins) of incubation and were also stable up to 65oC, showing the remaining activities of more than 70%. Samples containing protease and lipase as de-staining agents were tested by their ability to remove blood and palm oil stains, with results proving they were effective. Overall, the study showed that crude fish visceral extract contains protease and lipase and is useful in removing household stains, and could substitute for commercial detergents.
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“The viscera organ, composed of the gut, liver, spleen, stomach, heart, and pancreas, contains functional compounds such as proteins, lipids, amino acids, vitamins, minerals, and omega-3 fatty acids [13, 14].”
From Effects of Various Decellularization Methods for the Development of Decellularized Extracellular Matrix from Tilapia (Oreochromis niloticus) Viscera (2024) · cites this paper as [13]
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Cites
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Research works printed but not linked: 4
- [3]3. Chaijaroen T, Thongruang C. 2016. Extraction, characterization and activity of digestive enzyme from Nile tilapia (Oreochromis niloticus) viscera waste. Intl Food Res J 23 (4): 1432-1438.
- [8]8. Gorguna S, Akpinar MA. 2012. Purification and characterization of lipase from the liver of Carp, Cyprinus carpio (1758), living in lake Todurge (Sivas, Turkiye). Turkish J Fish Aquat Sci 12: 207-215. DOI: 10.4194/1303-2712-v12_2_03 · DOI
- [18]18. Mardina V and Yusof F. 2016. Purification and characterization of surfactant stable-protease from Bacillus licheniformis: a potential additive for laundry detergent. Int J Adv Biotechnol Res 7 (2): 634- 643. http://www.bipublication.com
- [21]21. Mardina V, Yusof F, Alam MZ. 2015. Statistical optimization of physicochemical factors for protease production by Bacillus licheniformis on skim latex serum fortified media J Eng Sci Technol 6 (1): 42-52.
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Aron, J., Bual, R., Alimasag, J., Arellano, F., Baclayon, L., Bantilan, Z. C., Lumancas, G., Nisperos, M. J., Labares, M., Valle, K. D. D., et al. (2024) · International Journal of Biomaterials · doi:10.1155/2024/6148496 · Cited by 7
Metadata
- Authors
- Mardina, V., Harmawan, T., Fitriani, F., SUFRIADI, E., Febriani, F., & Yusof, F.
- Affiliations
- Samudra University (ID)
- Universitas Syiah Kuala (ID)
- International Islamic University Malaysia (MY)
- Venue
- Biodiversitas Journal of Biological Diversity
- Publisher
- UNS Solo
- Published
- 2020-12-01
- Volume
- 21
- Issue
- 12
- ISSN
- 1412-033X (ISSN-L)
- 2085-4722
- OpenAlex
- W3116568370
- Cited by
- 8
- References
- 27
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 5 · 2023: 1
- FWCI
- 0.13
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Biodiversitas Journal of Biological Diversity (publishedVersion)
- The International Islamic University Malaysia Repository (The International Islamic University Malaysia) (acceptedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Physical Sciences › Chemistry › Organic Chemistry › Tannin, Tannase and Anticancer Activities
- Keywords
- Euthynnus affinis, fish viscera, proteolytic enzymes, lipase, enzyme characterization, stain removal, blood stain removal, enzyme purification, enzyme stability
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- CoChat. (2026). Citation check: Euthynnus affinis viscera-an alternative source for protease and lipase enzymes: Characteristic and potential application as destainer agent: Euthynnus affinis viscera as source for protease and lipase enzyme. https://app.cochat.ai/papers/10.13057/biodiv/d211250
- The paper
- Mardina, V., Harmawan, T., Fitriani, F., SUFRIADI, E., Febriani, F., & Yusof, F. (2020). Euthynnus affinis viscera-an alternative source for protease and lipase enzymes: Characteristic and potential application as destainer agent: Euthynnus affinis viscera as source for protease and lipase enzyme. Biodiversitas Journal of Biological Diversity. https://doi.org/10.13057/biodiv/d211250
