The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Cytotoxic effect of γ-sitosterol from Kejibeling (Strobilanthes crispus) and its mechanism of action towards c-myc gene expression and apoptotic pathway
Open accessAbstract
Background: This study aimed to analyze the cytotoxicity effect of γ-sitosterol isolated from “Kejibeling” (Strobilanthes crispus), a medicinal plant, on several cancer cell lines. The mechanisms of the effects were studied through the expression of cancer-caused gene, c-myc and apoptotic pathways.Methods: This in vitro study was done using human colon cancer cell lines (Caco-2), liver cancer cell lines (HepG2), hormone-dependent breast cancer cell lines (MCF-7) and the normal liver cell lines (Chang Liver). The cytotoxic effect was measured through MTT assay and the potential cytotoxic value was calculated by determining the toxic concentration which may kill up to 50% of the total cell used (IC50). Meanwhile, the cytotoxic mechanism was studied by determining the effect of adding γ-sitosterol to the c-myc gene expression by reverse transciptase-polymerase chain reaction (RT-PCR). The effect of γ-sitosterol through apoptotic pathway was studied by using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay.Results: γ-sitosterol was cytotoxic against Caco-2, HepG2, and MCF-7 with IC50-values of 8.3, 21.8, and 28.8 μg/mL, respectively. There were no IC50-values obtained from this compound against Chang Liver cell line. This compound induced apotosis on Caco-2 and HepG2 cell lines and suppressed the c-myc genes expression in both cells.Conclusion: γ-sitosterol was cytotoxic against colon and liver cancer cell lines and the effect was mediated by down-regulation of c-myc expression and induction of the apoptotic pathways.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The check wasn’t confident either way, so it gives no verdict — never disputed.
In the citing paper
“Second, 2-vinyl-4h-1,3-dithiine, a member of dithiins compound34.”
From Bioactivities of Allium longicuspis Regel against anthracnose of mango caused by Colletotrichum gloeosporioides (Penz.) (2020) · cites this paper as [34]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Nicoletti, I., Migliorati, G., Pagliacci, M. C., Grignani, F., & Riccardi, C. (1991) · Journal of Immunological Methods · doi:10.1016/0022-1759(91)90198-o · Cited by 4,562
Dang, C. V. (2012) · Cell · doi:10.1016/j.cell.2012.03.003 · Cited by 3,390
Thymocyte apoptosis induced by p53-dependent and independent pathways
Clarke, A. R., Purdie, C. A., Harrison, D. J., Morris, R. G., Bird, C. C., Hooper, M. L., & Wyllie, A. H. (1993) · Nature · doi:10.1038/362849a0 · Cited by 2,271
Brummitt, R. K., & POWELL, C. E. (1992) · Cited by 894
Gold, R., Schmied, M. C., Giegerich, G., Breitschopf, H., Hartung, H., Toyka, K. V., & Lassmann, H. (1994) · PubMed · Cited by 675
The role of DNA fragmentation in apoptosis
Bortner, C. D., Oldenburg, N. B. E., & Cidlowski, J. A. (1995) · Trends in Cell Biology · doi:10.1016/s0962-8924(00)88932-1 · Cited by 618
Gardens, K. R. B., POWELL, C. E., & Brummitt, R. K. (1992) · Royal Botanic Gardens, Kew eBooks · Cited by 517
Lane, D. P. (1993) · Nature · doi:10.1038/362786a0 · Cited by 340
Evans, D. L. I., & Dive, C. (1993) · PubMed · Cited by 146
Population-based cancer registration in Indonesia.
Wahidin, M., Noviani, R., Hermawan, S., Andriani, V., Ardian, A., & Djarir, H. (2012) · Asian Pacific Journal of Cancer Prevention · doi:10.7314/apjcp.2012.13.4.1709 · Cited by 78
Cited by
Sorted by citations
Siew, Y.-Y., Yew, H.-C., Neo, S.-Y., Seow, S.-V., Lew, S.-M., Lim, S.-W., Lim, C. S. E.-S., Ng, Y.-C., Seetoh, W.-G., Ali, A., et al. (2018) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2018.12.040 · Cited by 55
Major Phytochemical asγ‐Sitosterol Disclosing and Toxicity Testing in Lagerstroemia Species
Sirikhansaeng, P., Tanee, T., Sudmoon, R., & Chaveerach, A. (2017) · Evidence-based Complementary and Alternative Medicine · doi:10.1155/2017/7209851 · Cited by 54
Khoury, M. E., Haykal, T., Hodroj, M. H., Najem, S. A., Sarkis, R., Taleb, R. I., & Rizk, S. E. (2020) · Cancers · doi:10.3390/cancers12020435 · Cited by 28
Yahya, M., Ginting, B., & Saidi, N. (2021) · Molecules · doi:10.3390/molecules26226915 · Cited by 28
Mangoba, M. A. A., & Alvindia, D. D. G. (2023) · Scientific Reports · doi:10.1038/s41598-023-33396-5 · Cited by 29
Metadata
- Authors
- Endrini, S., Rahmat, A., Ismail, P., & Taufiq-Yap, Y. H.
- Affiliations
- Yarsi University (ID)
- Universiti Putra Malaysia (MY)
- Venue
- Medical Journal of Indonesia
- Published
- 2015-01-27
- Volume
- 23
- Issue
- 4
- Pages
- 203-8
- ISSN
- 0853-1773 (ISSN-L)
- 2252-8083
- OpenAlex
- W1987044059
- Cited by
- 43
- References
- 18
- Citations by year
- 2025: 9 · 2024: 3 · 2023: 9 · 2022: 4 · 2021: 6 · 2020: 7 · 2019: 2 · 2018: 1 · 2017: 1 · 2015: 1
- FWCI
- 0.43
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Medical Journal of Indonesia (publishedVersion, cc-by-nc)
- Medical Journal of Indonesia (submittedVersion, cc-by-nc)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Physical Sciences › Chemistry › Organic Chemistry › Phytochemical Studies and Bioactivities
- Physical Sciences › Chemistry › Organic Chemistry › Natural product bioactivities and synthesis
- Physical Sciences › Materials Science › Biomaterials › Phytochemistry and Bioactive Compounds
- Keywords
- γ-sitosterol, Strobilanthes crispus, cytotoxicity, c-myc expression, apoptosis, anticancer activity, colon cancer, liver cancer, breast cancer, medicinal plants, phytochemicals
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
