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Technical Aspects of Immunohistochemistry

Ramos-Vara, J. A.·2005·Veterinary Pathology

Abstract

Immunohistochemistry is an integral technique in many veterinary laboratories for diagnostic and research purposes. In the last decade, the ability to detect antigens (Ags) in tissue sections has improved dramatically, mainly by countering the deleterious effects of formaldehyde with antigen retrieval (AR) and increasing sensitivity of the detection systems. In this review, I address these topics and provide an overview of technical aspects of immunohistochemistry, including those related to antibodies (Abs) and Ags, fixation, AR, detection methods, background, and troubleshooting. Microarray technology and the use of rabbit monoclonal Abs in immunohistochemistry are also discussed.

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Metadata

Authors
Ramos-Vara, J. A.
Venue
Veterinary Pathology
Published
2005-07
Volume
42
Issue
4
Pages
405-426
ISSN
  • 0300-9858 (ISSN-L)
  • 1544-2217
OpenAlex
W2162445773
Cited by
905
References
1
FWCI
9.28
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Technical Aspects of Immunohistochemistry. https://app.cochat.ai/papers/10.1354/vp.42-4-405
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Ramos-Vara, J. A. (2005). Technical Aspects of Immunohistochemistry. Veterinary Pathology. https://doi.org/10.1354/vp.42-4-405

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