Importance of Membrane- or Matrix-Associated Forms of M-CSF and RANKL/ODF in Osteoclastogenesis Supported by SaOS-4/3 Cells Expressing Recombinant PTH/PTHrP Receptors

Itoh, Kanami; Udagawa, Nobuyuki; Matsuzaki, Kenichiro; Takami, Masamichi; Amano, Hitoshi; Shinki, Toshimasa; Ueno, Yutaka; Takahashi, Naoyuki; Suda, Tatsuo

doi:10.1359/jbmr.2000.15.9.1766

Importance of Membrane- or Matrix-Associated Forms of M-CSF and RANKL/ODF in Osteoclastogenesis Supported by SaOS-4/3 Cells Expressing Recombinant PTH/PTHrP Receptors

SaOS-4/3, a subclone of the human osteosarcoma cell line SaOS-2, established by transfecting the human parathyroid hormone/parathyroid hormone-related protein (PTH/PTHrP) receptor complementary DNA (cDNA), supported osteoclast formation in response to PTH in coculture with mouse bone marrow cells…