自组装制备Fe3O4@Au复合纳米粒子用于固定化葡萄糖氧化酶
Open accessAbstract
用化学共沉淀法合成了6~12 nm的超顺磁性Fe3O4纳米晶体, 在室温下用3-氨丙基三乙氧基硅烷(APTES)对其表面氨基化, 然后加入Frens法合成的金溶胶, 自组装制备了磁性Fe3O4@Au复合纳米粒子. 用透射电子显微镜(TEM)、紫外可见吸收光谱(UV-Vis)、傅里叶变换红外光谱(FT-IR)、震动样品磁场计(VSM)等方法对合成的金磁微粒的表面形貌、光学、结构、磁性质等进行表征. 结果表明, 合成的金磁微粒粒径分布均匀, 在15~20 nm, 磁响应性好. 金磁微粒有超顺磁性和易与生物分子结合的特点, 以葡萄糖氧化酶(GOx)为模型, 详细研究了固定化酶条件及固定化酶的酶学性质. 固定化酶的最优条件为: Fe3O4:HAuCl4摩尔比为0.5:1, pH 5.5, 温度为28℃. 固定化后葡萄糖氧化酶耐热性提高, 保存时间延长, 且能在外部磁场下分离反复使用.
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Prior to recent descriptions, no certain pre-Kimmeridgian representatives of maniraptorans were known.
“Prior to the recent description of abundant maniraptoran theropods from the Oxfordian of China ([4, 5, 27, 31, 34, 45, 46, 57, 58]), no certain pre-Kimmeridgian representatives of this clade were known.”
From Re-evaluation of the Haarlem Archaeopteryx and the radiation of maniraptoran theropod dinosaurs (2017) · cites this paper as [31]
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- Authors
- 杨, 婉., 王, 显., 黄, 硕., & 单, 志.
- Venue
- Chinese Science Bulletin
- Publisher
- Science China Press., Co. Ltd.
- Published
- 2009-02-01
- Volume
- 54
- Issue
- 4
- Pages
- 430-435
- ISSN
- 0023-074X (ISSN-L)
- OpenAlex
- W2804408860
- Cited by
- 23
- References
- 0
- Citations by year
- 2019: 1 · 2018: 3 · 2017: 5 · 2016: 1 · 2015: 1 · 2013: 4 · 2012: 1
- FWCI
- 1.07
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Chinese Science Bulletin (Chinese Version) (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
- Physical Sciences › Materials Science › Materials Chemistry › Magnetic Properties and Synthesis of Ferrites
- Keywords
- glucose oxidase immobilization, magnetic nanoparticles, superparamagnetism, self-assembly, gold sol, enzyme reusability
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