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Properties of a Defocused Optical System*
Abstract
The optical properties of an aberration-free defocused optical system used to image incoherently illuminated objects are analyzed. The exact diffraction optical transfer function (OTF) and point spread function (PSF) are compared to the geometrical OTF and PSF, respectively, for both small and large amounts of defocusing. It follows that geometrical optics does not describe diffraction optics very well for any reasonable amount of defocusing. Calculation of the diffraction OTF is complicated and time consuming, even with a large computer. An empirically derived analytic approximation to the diffraction OTF which is much easier to calculate, is formulated. It is also shown, and examples are presented to demonstrate, that the exact OTF and PSF are different in planes at equal distances on the two sides of the in-focus plane.
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Cited by
Chapter 13 Fluorescence Microscopy in Three Dimensions
Agard, D. A., Hiraoka, Y., Shaw, P., & Sedat, J. W. (1989) · Methods in Cell Biology · doi:10.1016/s0091-679x(08)60986-3 · Cited by 649
High-definition imaging using line-illumination modulation microscopy
Zhong, Q., Li, A., Jin, R., Zhang, D., Li, X., Jia, X., Ding, Z., Luo, P., Zhou, C., Jiang, C., et al. (2021) · Nature Methods · doi:10.1038/s41592-021-01074-x · Cited by 171
Metadata
- Authors
- Stokseth, P. A.
- Venue
- Journal of the Optical Society of America
- Publisher
- Optica Publishing Group
- Published
- 1969-10-01
- Volume
- 59
- Issue
- 10
- Pages
- 1314
- ISSN
- 0030-3941 (ISSN-L)
- OpenAlex
- W1983078517
- Cited by
- 305
- References
- 0
- FWCI
- 3.96
- Type
- journal article
- Language
- en
- Open access
- closed
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- Stokseth, P. A. (1969). Properties of a Defocused Optical System*. Journal of the Optical Society of America. https://doi.org/10.1364/josa.59.001314
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