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Ultra-compact fiber-optic two-photon microscope for functional fluorescence imaging in vivo
Open accessAbstract
We present a small, lightweight two-photon fiberscope and demonstrate its suitability for functional imaging in the intact brain. Our device consists of a hollow-core photonic crystal fiber for efficient delivery of near-IR femtosecond laser pulses, a spiral fiber-scanner for resonant beam steering, and a gradient-index lens system for fluorescence excitation, dichroic beam splitting, and signal collection. Fluorescence light is remotely detected using a standard photomultiplier tube. All optical components have 1 mm dimensions and the microscope's headpiece weighs only 0.6 grams. The instrument achieves micrometer resolution at frame rates of typically 25 Hz with a field-of-view of up to 200 microns. We demonstrate functional imaging of calcium signals in Purkinje cell dendrites in the cerebellum of anesthetized rats. The microscope will be easily portable by a rat or mouse and thus should enable functional imaging in freely behaving animals.
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Cited by
Miniaturized integration of a fluorescence microscope
Ghosh, K. K., Burns, L. D., Cocker, E. D., Nimmerjahn, A., Ziv, Y., Gamal, A. E., & Schnitzer, M. J. (2011) · Nature Methods · doi:10.1038/nmeth.1694 · Cited by 1,120
On the development of optical peripheral nerve interfaces
Anderson, H., & Weir, R. F. (2019) · Neural Regeneration Research · doi:10.4103/1673-5374.245461 · Cited by 14
Metadata
- Authors
- Engelbrecht, C. J., Johnston, R. S., Seibel, E. J., & Helmchen, F.
- Venue
- Optics Express
- Publisher
- Optica Publishing Group
- Published
- 2008-04-04
- Volume
- 16
- Issue
- 8
- Pages
- 5556
- ISSN
- 1094-4087 (ISSN-L)
- OpenAlex
- W2070737749
- Cited by
- 279
- References
- 0
- FWCI
- 11.76
- Type
- journal article
- Language
- en
- Open access
- gold
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- CoChat. (2026). Citation check: Ultra-compact fiber-optic two-photon microscope for functional fluorescence imaging in vivo. https://app.cochat.ai/papers/10.1364/oe.16.005556
- The paper
- Engelbrecht, C. J., Johnston, R. S., Seibel, E. J., & Helmchen, F. (2008). Ultra-compact fiber-optic two-photon microscope for functional fluorescence imaging in vivo. Optics Express. https://doi.org/10.1364/oe.16.005556
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
