The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
STED microscopy with a supercontinuum laser source
Open accessAbstract
We report on a straightforward yet powerful implementation of stimulated emission depletion (STED) fluorescence microscopy providing subdiffraction resolution in the far-field. Utilizing the same super-continuum pulsed laser source both for excitation and STED, this implementation of STED microscopy avoids elaborate preparations of laser pulses and conveniently provides multicolor imaging. Operating at pulse repetition rates around 1 MHz, it also affords reduced photobleaching rates by allowing the fluorophore to relax from excitable metastable dark states involved in photodegradation. The imaging of dense nanoparticles and of the microtubular network of mammalian cells evidences a spatial resolution of 30-50 nm in the focal plane, i.e. by a factor of 8-9 beyond the diffraction barrier.
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Citations
Cites
Hell, S. W. (2007) · Science · doi:10.1126/science.1137395 · Cited by 2,967
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Cited by
Stimulated Emission Depletion Microscopy
Blom, H., & Widengren, J. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00653 · Cited by 339
Two-photon excitation STED microscopy
Moneron, G., & Hell, S. W. (2009) · Optics Express · doi:10.1364/oe.17.014567 · Cited by 238
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Metadata
- Authors
- Wildanger, D., Rittweger, E., Kastrup, L., & Hell, S. W.
- Venue
- Optics Express
- Publisher
- Optica Publishing Group
- Published
- 2008-06-13
- Volume
- 16
- Issue
- 13
- Pages
- 9614
- ISSN
- 1094-4087 (ISSN-L)
- OpenAlex
- W2078085621
- Cited by
- 277
- References
- 1
- FWCI
- 9.69
- Type
- journal article
- Language
- en
- Open access
- gold
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- Wildanger, D., Rittweger, E., Kastrup, L., & Hell, S. W. (2008). STED microscopy with a supercontinuum laser source. Optics Express. https://doi.org/10.1364/oe.16.009614
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