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Cell damage in near-infrared multimode optical traps as a result of multiphoton absorption
Open accessAbstract
We report on cell damage of single cells confined in continuous-wave (cw), near-infrared (NIR) multimode optical traps as a result of multiphoton absorption phenomena. Trapping beams at NIR wavelengths less than 800 nm are capable of damaging cells through a two-photon absorption process. Cell damage is more pronounced in multimode cw traps compared with single-frequency true cw NIR traps because of transient power enhancement by longitudinal mode beating. Partial mode locking in tunable cw Ti:sapphire lasers used as trapping beam sources can produce unstable subnanosecond pulses at certain wavelengths that amplify multiphoton absorption effects significantly. We recommend the use of single-frequency long-wavelength NIR trapping beams for optical micromanipulation of vital cells.
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Citations
Cited by
Multiphoton microscopy in life sciences
König, K. (2000) · Journal of Microscopy · doi:10.1046/j.1365-2818.2000.00738.x · Cited by 1,265
Self-citationOptical tweezers for single cells
Zhang, H., & Liu, K.-K. (2008) · Journal of The Royal Society Interface · doi:10.1098/rsif.2008.0052 · Cited by 768
Characterization of Photodamage to Escherichia coli in Optical Traps
Neuman, K. C., Chadd, E. H., Liou, G. F., Bergman, K., & Block, S. M. (1999) · Biophysical Journal · doi:10.1016/s0006-3495(99)77117-1 · Cited by 687
Highly Nonlinear Photodamage in Two-Photon Fluorescence Microscopy
Hopt, A., & Neher, E. (2001) · Biophysical Journal · doi:10.1016/s0006-3495(01)76173-5 · Cited by 442
Surgical applications of femtosecond lasers
Chung, S. H., & Mazur, E. (2009) · Journal of Biophotonics · doi:10.1002/jbio.200910053 · Cited by 281
Metadata
- Authors
- König, K., Liang, H., Berns, M. W., & Tromberg, B. J.
- Venue
- Optics Letters
- Publisher
- Optica Publishing Group
- Published
- 1996-07-15
- Volume
- 21
- Issue
- 14
- Pages
- 1090
- ISSN
- 0146-9592 (ISSN-L)
- 1539-4794
- OpenAlex
- W2016952847
- Cited by
- 158
- References
- 0
- FWCI
- 3.84
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Cell damage in near-infrared multimode optical traps as a result of multiphoton absorption. https://app.cochat.ai/papers/10.1364/ol.21.001090
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- König, K., Liang, H., Berns, M. W., & Tromberg, B. J. (1996). Cell damage in near-infrared multimode optical traps as a result of multiphoton absorption. Optics Letters. https://doi.org/10.1364/ol.21.001090
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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