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Fluorescence Intensity Calibration Using the Raman Scatter Peak of Water
Abstract
Fluorescence data of replicate samples obtained from different fluorescence spectrometers or by the same spectrometer but with different instrument settings can have great intensity differences. In order to compare such data an intensity calibration must be applied. Here we explain a simple calibration method for fluorescence intensity using only the integrated area of a water Raman peak. By applying this method to data from three different instruments, we show that it is possible to remove instrument-dependent intensity factors, and we present results on a unified scale of Raman units. The method presented is a rapid and simple approach suitable for routine measurements with no need for hazardous chemicals.
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Cited by
Shutova, Y., Baker, A., Bridgeman, J., & Henderson, R. K. (2014) · Water Research · doi:10.1016/j.watres.2014.01.053 · Cited by 438
Lee, Y. K., Murphy, K. R., & Hur, J. (2020) · Environmental Science & Technology · doi:10.1021/acs.est.0c00942 · Cited by 380
Lavonen, E. E., Kothawala, D. N., Tranvik, L. J., Gonsior, M., Schmitt-Kopplin, P., & Köhler, S. J. (2015) · Water Research · doi:10.1016/j.watres.2015.08.024 · Cited by 274
Yang, X., Tsibart, A., Nam, H., Hur, J., El-Naggar, A., Tack, F. M. G., Wang, C.-H., Lee, Y. H., Tsang, D. C. W., & Ok, Y. S. (2019) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2018.11.042 · Cited by 205
Drinking water treatment response following a Colorado wildfire
Hohner, A. K., Cawley, K., Oropeza, J., Summers, R. S., & Rosario-Ortiz, F. L. (2016) · Water Research · doi:10.1016/j.watres.2016.08.034 · Cited by 141
Metadata
- Authors
- Lawaetz, A. J., & Stedmon, C. A.
- Venue
- Applied Spectroscopy
- Publisher
- SAGE Publications
- Published
- 2009-08
- Volume
- 63
- Issue
- 8
- Pages
- 936-940
- ISSN
- 0003-7028 (ISSN-L)
- 1943-3530
- OpenAlex
- W1999760584
- Cited by
- 1,151
- References
- 0
- FWCI
- 7.12
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
