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Cited by 271Cellular Mechanics and InteractionsDevelopmental Biology and Gene RegulationMicrotubule and mitosis dynamics

Control of Directed Cell Migration In Vivo by Membrane-to-Cortex Attachment

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Diz-Muñoz, Alba, Krieg, Michael, Bergert, Martin, Ibarlucea-Benitez, Itziar, Muller, Daniel J., Paluch, Ewa, Heisenberg, Carl-Philipp·2010·PLoS Biology
DOI 10.1371/journal.pbio.1000544PMC PMC2994655
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Abstract

Cell shape and motility are primarily controlled by cellular mechanics. The attachment of the plasma membrane to the underlying actomyosin cortex has been proposed to be important for cellular processes involving membrane deformation. However, little is known about the actual function of membrane-to-cortex attachment (MCA) in cell protrusion formation and migration, in particular in the context of the developing embryo. Here, we use a multidisciplinary approach to study MCA in zebrafish mesoderm and endoderm (mesendoderm) germ layer progenitor cells, which migrate using a combination of different protrusion types, namely, lamellipodia, filopodia, and blebs, during zebrafish gastrulation. By interfering with the activity of molecules linking the cortex to the membrane and measuring resulting changes in MCA by atomic force microscopy, we show that reducing MCA in mesendoderm progenitors increases the proportion of cellular blebs and reduces the directionality of cell migration. We propose that MCA is a key parameter controlling the relative proportions of different cell protrusion types in mesendoderm progenitors, and thus is key in controlling directed migration during gastrulation.

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  • Alternative perturbations such as mechanical stretching, RhoA-ROCK activation, or modification of lipid composition can induce significant disruption ranging from membrane-cytoskeleton decoupling, contractility-induced retraction to altered lipid composition and gene expression.

    “Alternative perturbations such as mechanical stretching73, RhoA-ROCK activation74–76, or the modification of lipid composition77 can induce significant disruption ranging from membrane-cytoskeleton decoupling, contractility-induced retraction to altered lipid composition and gene expression.”

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Authors
Diz-Muñoz, A., Krieg, M., Bergert, M., Ibarlucea-Benitez, I., Muller, D. J., Paluch, E., & Heisenberg, C.-P.
Affiliations
  • International Institute of Molecular and Cell Biology (PL)
  • Biotechnologisches Zentrum (DE)
  • Institute of Science and Technology Austria (AT)
Corresponding author
  • Krieg, Michael
  • Paluch, Ewa
  • Heisenberg, Carl-Philipp
ORCID
Venue
PLoS Biology
Publisher
Public Library of Science (PLoS)
Published
2010-11-30
Volume
8
Issue
11
Pages
e1000544
ISSN
  • 1544-9173 (ISSN-L)
  • 1545-7885
DOI
10.1371/journal.pbio.1000544
PMC
PMC2994655
OpenAlex
W2145865462
Cited by
271
References
0
Citations by year
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FWCI
6.01
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS Biology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Max Planck Institute for Plasma Physics (submittedVersion)
  • UCL Discovery (University College London) (submittedVersion)
  • Figshare (submittedVersion, cc-by)
  • MPG.PuRe (Max Planck Society) (submittedVersion)
  • Qucosa (Saxon State and University Library Dresden) (submittedVersion, cc-by)
  • Repository for Publications and Research Data (ETH Zurich) (submittedVersion, cc-by)
  • ETH Zürich Research Collection (cc-by)
Funders
  • Institute of Science and Technology Austria
  • Boehringer Ingelheim Fonds
  • Deutsche Forschungsgemeinschaft (PA 1590/1-1, 1590/1-1, HE 3231/6-1)
  • Max-Planck-Gesellschaft
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords
cell migration, cell protrusions, blebs, lamellipodia, filopodia, zebrafish gastrulation, actomyosin cortex, cell shape, migration directionality, atomic force microscopy
MeSH terms
Animals, Cell Membrane, Cell Movement, Cytoskeleton, Mesoderm, Pseudopodia, Stem Cells, Zebrafish, Microscopy, Confocal, Microscopy, Atomic Force, Gastrulation

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