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Cited by 315Neurobiology and Insect Physiology ResearchInsect symbiosis and bacterial influencesGut microbiota and health

Commensal bacteria and essential amino acids control food choice behavior and reproduction

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Leitão-Gonçalves, Ricardo, Carvalho-Santos, Zita, Francisco, Ana Patrícia, Fioreze, Gabriela Tondolo, Anjos, Margarida, Baltazar, Célia, Elias, Ana Paula, Itskov, Pavel M. +2 more·2017·PLOS Biology
DOI 10.1371/journal.pbio.2000862PMC PMC5404834
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Abstract

Choosing the right nutrients to consume is essential to health and wellbeing across species. However, the factors that influence these decisions are poorly understood. This is particularly true for dietary proteins, which are important determinants of lifespan and reproduction. We show that in Drosophila melanogaster, essential amino acids (eAAs) and the concerted action of the commensal bacteria Acetobacter pomorum and Lactobacilli are critical modulators of food choice. Using a chemically defined diet, we show that the absence of any single eAA from the diet is sufficient to elicit specific appetites for amino acid (AA)-rich food. Furthermore, commensal bacteria buffer the animal from the lack of dietary eAAs: both increased yeast appetite and decreased reproduction induced by eAA deprivation are rescued by the presence of commensals. Surprisingly, these effects do not seem to be due to changes in AA titers, suggesting that gut bacteria act through a different mechanism to change behavior and reproduction. Thus, eAAs and commensal bacteria are potent modulators of feeding decisions and reproductive output. This demonstrates how the interaction of specific nutrients with the microbiome can shape behavioral decisions and life history traits.

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  • In Drosophila, giving GF flies Acetobacter pomorum and Lactobacillus spp. changed their overall food preference.

    “However, an intriguing study in Drosophila points to a key role for the microbiota in food choice behavior, as when GF flies were given Acetobacter pomorum and Lactobacillus spp., it changed their overall food preference (Leitao-Goncalves et al., 2017).”

    From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Leitao-Goncalves et al., 2017

    The Results section, “Acetobacter pomorum acts together with Lactobacilli to modify food choice” (page unavailable), reports that adding A. pomorum with either L. plantarum or L. brevis suppressed yeast appetite in essential-amino-acid-deprived flies. The Methods confirm that these experiments used axenic or gnotobiotic flies. The claim’s “overall food preference” is broader than the specific, deprivation-dependent yeast preference the paper tested. (LLM, conf=0.85)

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References 96 · Cited by 315

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Authors
Leitão-Gonçalves, R., Carvalho-Santos, Z., Francisco, A. P., Fioreze, G. T., Anjos, M., Baltazar, C., Elias, A. P., Itskov, P. M., Piper, M. D. W., & Ribeiro, C.
Affiliations
  • Centro Champalimaud (PT)
  • Monash University (AU)
Corresponding author
Ribeiro, Carlos
ORCID
Venue
PLOS Biology
Publisher
Public Library of Science (PLoS)
Published
2017-04-25
Volume
15
Issue
4
Pages
e2000862
ISSN
  • 1544-9173 (ISSN-L)
  • 1545-7885
DOI
10.1371/journal.pbio.2000862
PMC
PMC5404834
OpenAlex
W2608401495
Cited by
315
References
96
Citations by year
2026: 27 · 2025: 28 · 2024: 25 · 2023: 37 · 2022: 28 · 2021: 39 · 2020: 45 · 2019: 36 · 2018: 35 · 2017: 15
FWCI
17.54
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS Biology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Monash University Research Portal (Monash University) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
Funders
  • European Commission (289941)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • Fundação Bial
  • Fundação Champalimaud
  • National Institutes of Health (NIH P40OD018537, P40OD018537)
  • Directorate for Biological Sciences
  • Biotechnology and Biological Sciences Research Council (BB/I011544/1)
  • Australian Research Council (FT150100237)
  • Fundação para a Ciência e a Tecnologia (SFRH/BPD/78947/2011, PTDC/BIA-BCM/118684/2010, SFRH/BPD/76201/2011)
  • Ciência sem Fronteiras
  • Institute of Genetics
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect symbiosis and bacterial influences
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords
commensal bacteria, essential amino acids, food choice, Drosophila melanogaster, Acetobacter pomorum, lactobacilli, amino acid starvation, reproduction, gut microbiota, nutrient sensing, feeding behavior, life-history traits
MeSH terms
Gastrointestinal Microbiome, Acetobacter, Amino Acids, Essential, Animals, Appetite Regulation, Behavior, Animal, Drosophila melanogaster, Feeding Behavior, Female, Food Preferences, Host-Parasite Interactions, Lactobacillus, Oviposition, Species Specificity, Enterococcus faecalis, Symbiosis, Yeast, Dried, Acetobacteraceae, Drosophila Proteins, Animals, Genetically Modified, Complex Mixtures, Gene Knockout Techniques

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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