CoChat
Sign Up
Cited by 1,221Forensic and Genetic ResearchGenetic diversity and population structureGenetic Associations and Epidemiology

The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.

Resolving Individuals Contributing Trace Amounts of DNA to Highly Complex Mixtures Using High-Density SNP Genotyping Microarrays

Open access
Homer, Nils, Szelinger, Szabolcs, Redman, Margot, Duggan, David, Tembe, Waibhav, Muehling, Jill, Pearson, John V., Stephan, Dietrich A., Nelson, Stanley F., Craig, David W.·2008·PLoS Genetics
DOI 10.1371/journal.pgen.1000167PMC PMC2516199
Open this paper in CoChat — free accountOpen PDF

Abstract

We use high-density single nucleotide polymorphism (SNP) genotyping microarrays to demonstrate the ability to accurately and robustly determine whether individuals are in a complex genomic DNA mixture. We first develop a theoretical framework for detecting an individual's presence within a mixture, then show, through simulations, the limits associated with our method, and finally demonstrate experimentally the identification of the presence of genomic DNA of specific individuals within a series of highly complex genomic mixtures, including mixtures where an individual contributes less than 0.1% of the total genomic DNA. These findings shift the perceived utility of SNPs for identifying individual trace contributors within a forensics mixture, and suggest future research efforts into assessing the viability of previously sub-optimal DNA sources due to sample contamination. These findings also suggest that composite statistics across cohorts, such as allele frequency or genotype counts, do not mask identity within genome-wide association studies. The implications of these findings are discussed.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1371/journal.pgen.1000167

Cites

21 of 21 references verified0 citation errors · 0 not checkable

Statistics

Figures

Figure 1 To give insight into the intuition behind our method, we present for a given SNP three different scenarios for the possible allele frequency of the person of interest corresponding to the genotypes AA, AB, and BB. The allele frequencies of the reference population, person of interest, and the mixture are described as Mi, Yi, and Popi respectively. We see that the distance measure is greater (and positive) when the Yi of the person of interest is closer to the Mi of the mixture than to the Popi of the reference population. Similarly, the distance measure is smaller (and negative) when the Yi of the person of interest is closer to the Popi of the reference population than to Mi of the mixture. Our test statistic is then the z-score using this distance measure.
Figure 1 To give insight into the intuition behind our method, we present for a given SNP three different scenarios for the possible allele frequency of the person of interest corresponding to the genotypes AA, AB, and BB. The allele frequencies of the reference population, person of interest, and the mixture are described as Mi, Yi, and Popi respectively. We see that the distance measure is greater (and positive) when the Yi of the person of interest is closer to the Mi of the mixture than to the Popi of the reference population. Similarly, the distance measure is smaller (and negative) when the Yi of the person of interest is closer to the Popi of the reference population than to Mi of the mixture. Our test statistic is then the z-score using this distance measure.CC BY · PubMed Central
Figure 2 Simulation Results. Using 1423 Wellcome Trust 58C individuals, we give log scaled p-values for simulations based on three variables: the number of SNPs (s), the fraction of the individual in the mixture (f), and the probe variance (vp). The graphs plot the relationships between the three variables with a different variable fixed in each graph. The log scaled p-values are represented by the color of each point in the graph, as well as the z-axis on the right graphs. These simulations suggest that we should be able to resolve mixtures where a given individual is 0.1% of the mixture (f), probe variance is at most 0.01 (vp) and the number of SNPs probed is 50,000 (s).
Figure 2 Simulation Results. Using 1423 Wellcome Trust 58C individuals, we give log scaled p-values for simulations based on three variables: the number of SNPs (s), the fraction of the individual in the mixture (f), and the probe variance (vp). The graphs plot the relationships between the three variables with a different variable fixed in each graph. The log scaled p-values are represented by the color of each point in the graph, as well as the z-axis on the right graphs. These simulations suggest that we should be able to resolve mixtures where a given individual is 0.1% of the mixture (f), probe variance is at most 0.01 (vp) and the number of SNPs probed is 50,000 (s).CC BY · PubMed Central
Figure 3 Experimental validation using a series of mixtures (see Methods A–F) assayed on the Affymetrix GeneChip 5.0, Illumina BeadArray 550 and the Illumina 450S Duo Human BeadChip. The x-axis shows each individual in the CEU HapMap population, the left y-axis shows the p-value (log scaled), and the right y-axis shows the value of the test statistic. For mixtures A, B, E and F those in the mixture are colored green and those not in the mixture are colored red. For mixtures C and D those individuals who are not in the mixtures are colored red, those individuals who are related to the 1% or 10% individuals in the mixtures are colored orange, those individuals who are related to the 90% or 99% are colored yellow, and those people in the mixture are colored green. In all mixtures, the identification of the presence of a person's genomic DNA was possible.
Figure 3 Experimental validation using a series of mixtures (see Methods A–F) assayed on the Affymetrix GeneChip 5.0, Illumina BeadArray 550 and the Illumina 450S Duo Human BeadChip. The x-axis shows each individual in the CEU HapMap population, the left y-axis shows the p-value (log scaled), and the right y-axis shows the value of the test statistic. For mixtures A, B, E and F those in the mixture are colored green and those not in the mixture are colored red. For mixtures C and D those individuals who are not in the mixtures are colored red, those individuals who are related to the 1% or 10% individuals in the mixtures are colored orange, those individuals who are related to the 90% or 99% are colored yellow, and those people in the mixture are colored green. In all mixtures, the identification of the presence of a person's genomic DNA was possible.CC BY · PubMed Central

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 21 references

Cited by

Sorted by citations

Items 701–800 of 1,000

Related

See all

Metadata

Authors
Homer, N., Szelinger, S., Redman, M., Duggan, D., Tembe, W., Muehling, J., Pearson, J. V., Stephan, D. A., Nelson, S. F., & Craig, D. W.
Affiliations
  • Translational Genomics Research Institute (US)
  • University of California, Los Angeles (US)
Corresponding author
Craig, David W.
ORCID
Venue
PLoS Genetics
Publisher
Public Library of Science (PLoS)
Published
2008-08-29
Volume
4
Issue
8
Pages
e1000167
ISSN
  • 1553-7390 (ISSN-L)
  • 1553-7404
DOI
10.1371/journal.pgen.1000167
PMC
PMC2516199
OpenAlex
W2040228409
Cited by
1,221
References
21
Citations by year
2026: 24 · 2025: 42 · 2024: 54 · 2023: 71 · 2022: 81 · 2021: 76 · 2020: 89 · 2019: 103 · 2018: 87 · 2017: 81 · 2016: 65 · 2015: 59 · 2014: 79 · 2013: 62 · 2012: 63
FWCI
34.85
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS Genetics (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Wellcome Trust (076113)
  • Translational Genomics Research Institute
  • National Institutes of Health
  • National Heart, Lung, and Blood Institute (U01HL086528)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Forensic and Genetic Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Associations and Epidemiology
Keywords
DNA mixture analysis, single nucleotide polymorphisms, forensic DNA identification, sample contamination, genome-wide association study
MeSH terms
Computer Simulation, Genetics, Medical, Genotype, Humans, Genome, Human, Oligonucleotide Array Sequence Analysis, Polymorphism, Single Nucleotide

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review