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Cited by 86melanin and skin pigmentationZebrafish Biomedical Research ApplicationsDevelopmental Biology and Gene Regulation

Basonuclin-2 requirements for zebrafish adult pigment pattern development and female fertility

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Lang, Michael R., Patterson, Larissa B., Gordon, Tiffany N., Johnson, Stephen L., Parichy, David M.·2009·PLoS Genetics
DOI 10.1371/journal.pgen.1000744PMC PMC2776513
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Abstract

Relatively little is known about the generation of adult form. One complex adult trait that is particularly amenable to genetic and experimental analysis is the zebrafish pigment pattern, which undergoes extensive remodeling during post-embryonic development to form adult stripes. These stripes result from the arrangement of three classes of neural crest-derived pigment cells, or chromatophores: melanophores, xanthophores, and iridophores. Here, we analyze the zebrafish bonaparte mutant, which has a normal early pigment pattern but exhibits a severe disruption to the adult stripe pattern. We show that the bonaparte mutant phenotype arises from mutations in basonuclin-2 (bnc2), encoding a highly conserved, nuclear-localized zinc finger protein of unknown function. We show that bnc2 acts non-autonomously to the melanophore lineage and is expressed by hypodermal cells adjacent to chromatophores during adult pigment pattern formation. In bonaparte (bnc2) mutants, all three types of chromatophores differentiate but then are lost by extrusion through the skin. We further show that while bnc2 promotes the development of two genetically distinct populations of melanophores in the body stripes, chromatophores of the fins and scales remain unaffected in bonaparte mutants, though a requirement of fin chromatophores for bnc2 is revealed in the absence of kit and colony stimulating factor-1 receptor activity. Finally, we find that bonaparte (bnc2) mutants exhibit dysmorphic ovaries correlating with infertility and bnc2 is expressed in somatic ovarian cells, whereas the related gene, bnc1, is expressed within oocytes; and we find that both bnc2 and bnc1 are expressed abundantly within the central nervous system. These findings identify bnc2 as an important mediator of adult pigment pattern formation and identify bonaparte mutants as an animal model for dissecting bnc2 functions.

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  • Stromal cells of the hypodermis and iridophores regulate melanophore and xanthophore numbers during adult pigment pattern formation.

    “stromal cells of the hypodermis (Lang et al., 2009) and also iridophores (Frohnhöfer et al., 2013; Patterson and Parichy, 2013) regulate melanophore and xanthophore numbers during adult pigment pattern formation”

    From Thyroid hormone regulates distinct paths to maturation in pigment cell lineages (2019) · cites this paper as Lang et al., 2009

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Authors
Lang, M. R., Patterson, L. B., Gordon, T. N., Johnson, S. L., & Parichy, D. M.
Affiliations
  • University of Washington (US)
  • Washington University in St. Louis (US)
Corresponding author
Parichy, David M.
ORCID
Venue
PLoS Genetics
Publisher
Public Library of Science (PLoS)
Published
2009-11-26
Volume
5
Issue
11
Pages
e1000744
ISSN
  • 1553-7390 (ISSN-L)
  • 1553-7404
DOI
10.1371/journal.pgen.1000744
PMC
PMC2776513
OpenAlex
W2077684781
Cited by
86
References
1
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FWCI
2.07
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS Genetics (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Institutes of Health
  • National Institute of General Medical Sciences (GM56988, R01GM062182, R01GM056988)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (T32HD007183)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
zebrafish, chromatophores, melanophores, xanthophores, iridophores, zinc finger proteins, female fertility, KIT signaling, colony-stimulating factor 1 receptor, postembryonic development
MeSH terms
Animals, Carrier Proteins, Female, Fertility, Melanophores, Mutation, Ovary, Pigmentation, Zebrafish, Zinc Fingers, Body Patterning, Zebrafish Proteins

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