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Cited by 61Bone Metabolism and DiseasesBone and Dental Protein StudiesGenomics and Chromatin Dynamics

Prdm5 Regulates Collagen Gene Transcription by Association with RNA Polymerase II in Developing Bone

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Galli, Giorgio Giacomo, Honnens de Lichtenberg, Kristian, Carrara, Matteo, Hans, Wolfgang, Wuelling, Manuela, Mentz, Bettina, Multhaupt, Hinke Arnolda, Fog, Cathrine Kolster +10 more·2012·PLoS Genetics
DOI 10.1371/journal.pgen.1002711PMC PMC3349747
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Abstract

PRDM family members are transcriptional regulators involved in tissue specific differentiation. PRDM5 has been reported to predominantly repress transcription, but a characterization of its molecular functions in a relevant biological context is lacking. We demonstrate here that Prdm5 is highly expressed in developing bones; and, by genome-wide mapping of Prdm5 occupancy in pre-osteoblastic cells, we uncover a novel and unique role for Prdm5 in targeting all mouse collagen genes as well as several SLRP proteoglycan genes. In particular, we show that Prdm5 controls both Collagen I transcription and fibrillogenesis by binding inside the Col1a1 gene body and maintaining RNA polymerase II occupancy. In vivo, Prdm5 loss results in delayed ossification involving a pronounced impairment in the assembly of fibrillar collagens. Collectively, our results define a novel role for Prdm5 in sustaining the transcriptional program necessary to the proper assembly of osteoblastic extracellular matrix.

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  • PRDM5 regulates collagen transcription and fibrillogenesis by binding collagen genes and maintaining RNA polymerase II occupancy.

    “More specifically, it regulates collagen transcription and fibrillogenesis by binding collagen genes and maintaining RNA polymerase II occupancy [Galli et al., 2012].”

    From The Ehlers–Danlos syndromes, rare types (2017) · cites this paper as Galli et al., 2012

  • In line with its role in BCS, PRDM5 was shown to regulate transcription of ECM genes, including several collagen genes and small leucine-rich proteoglycans (SLRP) in pre-osteoblastic mouse cells.

    “In line with its role in BCS, PRDM5 was shown to regulate transcription of ECM genes, including several collagen genes and small leucine-rich proteoglycans (SLRP) in pre-osteoblastic mouse cells.”

    From The Ehlers–Danlos syndromes, rare types (2017) · cites this paper as Galli et al., 2012

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References 41 · Cited by 61

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Authors
Galli, G. G., Honnens de Lichtenberg, K., Carrara, M., Hans, W., Wuelling, M., Mentz, B., Multhaupt, H. A., Fog, C. K., Jensen, K. T., Rappsilber, J., Vortkamp, A., Coulton, L., Fuchs, H., Gailus-Durner, V., Hrabě de Angelis, M., Calogero, R. A., Couchman, J. R., & Lund, A. H.
Affiliations
  • University of Copenhagen (DK)
  • University of Turin (IT)
  • Helmholtz Munich (DE)
  • University of Duisburg-Essen (DE)
  • Wellcome Centre for Cell Biology (GB)
  • University of Sheffield (GB)
Corresponding author
Lund, Anders Henrik
ORCID
Venue
PLoS Genetics
Publisher
Public Library of Science (PLoS)
Published
2012-05-10
Volume
8
Issue
5
Pages
e1002711
ISSN
  • 1553-7390 (ISSN-L)
  • 1553-7404
DOI
10.1371/journal.pgen.1002711
PMC
PMC3349747
OpenAlex
W2125831529
Cited by
61
References
41
Citations by year
2026: 3 · 2025: 4 · 2024: 2 · 2023: 3 · 2022: 5 · 2021: 7 · 2020: 4 · 2019: 1 · 2018: 2 · 2017: 4 · 2016: 3 · 2015: 8 · 2014: 3 · 2013: 11 · 2012: 1
FWCI
2.96
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS Genetics (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • White Rose Research Online (University of Leeds, The University of Sheffield, University of York) (acceptedVersion)
  • Institutional Research Information System University of Turin (University of Turin) (submittedVersion, other-oa)
  • Edinburgh Research Explorer (University of Edinburgh) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
  • mediaTUM (Technical University of Munich) (submittedVersion, cc-by)
  • Site cant be reached (submittedVersion, cc-by)
  • Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
Funders
  • Kræftens Bekæmpelse
  • Højteknologifonden
  • Wellcome Trust (JR 084229, 077707, 091020, 084229, 092076, 201102)
  • INFRAFRONTIER (01KX1012)
  • National Research Foundation
  • Danmarks Grundforskningsfond
  • Bundesministerium für Bildung und Forschung (01GS0850, 01KX1012)
  • Lundbeckfonden
  • Novo Nordisk
  • Novo Nordisk Fonden
  • H. Lundbeck A/S
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
PRDM5, RNA polymerase II, type I collagen, COL1A1, fibril formation, osteoblasts, ossification, extracellular matrix assembly, proteoglycans, transcriptional repression, skeletal development
MeSH terms
Collagen Type I, alpha 1 Chain, Animals, Bone Development, Cell Differentiation, DNA-Binding Proteins, Enhancer Elements, Genetic, Extracellular Matrix, Organ Specificity, Osteoblasts, Promoter Regions, Genetic, Proteoglycans, RNA Polymerase II, Transcription Factors, Transcription, Genetic, 3T3 Cells, Genome, Gene Expression Regulation, Developmental, Fibrillar Collagens, Collagen Type I, Embryonic Development, Mice, Decorin

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