The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Taxonomic Reliability of DNA Sequences in Public Sequence Databases: A Fungal Perspective
Open accessAbstract
BACKGROUND: DNA sequences are increasingly seen as one of the primary information sources for species identification in many organism groups. Such approaches, popularly known as barcoding, are underpinned by the assumption that the reference databases used for comparison are sufficiently complete and feature correctly and informatively annotated entries. METHODOLOGY/PRINCIPAL FINDINGS: The present study uses a large set of fungal DNA sequences from the inclusive International Nucleotide Sequence Database to show that the taxon sampling of fungi is far from complete, that about 20% of the entries may be incorrectly identified to species level, and that the majority of entries lack descriptive and up-to-date annotations. CONCLUSIONS: The problems with taxonomic reliability and insufficient annotations in public DNA repositories form a tangible obstacle to sequence-based species identification, and it is manifest that the greatest challenges to biological barcoding will be of taxonomical, rather than technical, nature.
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Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Altschul, S. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.17.3389 · Cited by 73,302
DNA barcoding: error rates based on comprehensive sampling
Meyer, C. P., & Paulay, G. (2005) · PLoS Biology · doi:10.1371/journal.pbio.0030422 · Cited by 1,957
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The Colletotrichum gloeosporioides species complex
Weir, B. S., Johnston, P. R., & Damm, U. (2012) · Studies in Mycology · doi:10.3114/sim0011 · Cited by 1,646
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Marine Fungi: Their Ecology and Molecular Diversity
Richards, T. A., Jones, M. D. M., Leonard, G., & Bass, D. (2012) · Annual Review of Marine Science · doi:10.1146/annurev-marine-120710-100802 · Cited by 478
Jumpponen, A., & Jones, K. L. (2009) · New Phytologist · doi:10.1111/j.1469-8137.2009.02990.x · Cited by 432
Metadata
- Authors
- Nilsson, R. H., Ryberg, M., Kristiansson, E., Abarenkov, K., Larsson, K.-H., & Kõljalg, U.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2006-12-20
- Volume
- 1
- Issue
- 1
- Pages
- e59
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2002861821
- Cited by
- 654
- References
- 2
- FWCI
- 15.41
- Type
- journal article
- Language
- en
- Open access
- gold
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- Nilsson, R. H., Ryberg, M., Kristiansson, E., Abarenkov, K., Larsson, K.-H., & Kõljalg, U. (2006). Taxonomic Reliability of DNA Sequences in Public Sequence Databases: A Fungal Perspective. PLoS ONE. https://doi.org/10.1371/journal.pone.0000059
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