The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Metabolic Engineering of Potato Carotenoid Content through Tuber-Specific Overexpression of a Bacterial Mini-Pathway
Open accessAbstract
BACKGROUND: Since the creation of "Golden Rice", biofortification of plant-derived foods is a promising strategy for the alleviation of nutritional deficiencies. Potato is the most important staple food for mankind after the cereals rice, wheat and maize, and is extremely poor in provitamin A carotenoids. METHODOLOGY: We transformed potato with a mini-pathway of bacterial origin, driving the synthesis of beta-carotene (Provitamin A) from geranylgeranyl diphosphate. Three genes, encoding phytoene synthase (CrtB), phytoene desaturase (CrtI) and lycopene beta-cyclase (CrtY) from Erwinia, under tuber-specific or constitutive promoter control, were used. 86 independent transgenic lines, containing six different promoter/gene combinations, were produced and analyzed. Extensive regulatory effects on the expression of endogenous genes for carotenoid biosynthesis are observed in transgenic lines. Constitutive expression of the CrtY and/or CrtI genes interferes with the establishment of transgenosis and with the accumulation of leaf carotenoids. Expression of all three genes, under tuber-specific promoter control, results in tubers with a deep yellow ("golden") phenotype without any adverse leaf phenotypes. In these tubers, carotenoids increase approx. 20-fold, to 114 mcg/g dry weight and beta-carotene 3600-fold, to 47 mcg/g dry weight. CONCLUSIONS: This is the highest carotenoid and beta-carotene content reported for biofortified potato as well as for any of the four major staple foods (the next best event being "Golden Rice 2", with 31 mcg/g dry weight beta-carotene). Assuming a beta-carotene to retinol conversion of 6ratio1, this is sufficient to provide 50% of the Recommended Daily Allowance of Vitamin A with 250 gms (fresh weight) of "golden" potatoes.
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Self-citationThe small, versatilepPZP family ofAgrobacterium binary vectors for plant transformation
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Carotenoids and their cleavage products: Biosynthesis and functions
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In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning
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Biofortified crops to alleviate micronutrient malnutrition
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Self-citationCrops that feed the world 8: Potato: are the trends of increased global production sustainable?
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Metadata
- Authors
- Diretto, G., Al-Babili, S., Tavazza, R., Papacchioli, V., Beyer, P., & Giuliano, G.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2007-04-04
- Volume
- 2
- Issue
- 4
- Pages
- e350
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W1995038549
- Cited by
- 366
- References
- 34
- FWCI
- 7.78
- Type
- journal article
- Language
- en
- Open access
- gold
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