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Cited by 275Cellular Mechanics and InteractionsCell Adhesion Molecules ResearchProtein Kinase Regulation and GTPase Signaling

Comparative dynamics of retrograde actin flow and focal adhesions: formation of nascent adhesions triggers transition from fast to slow flow

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Alexandrova, Antonina Y., Arnold, Katya, Schaub, Sébastien, Vasiliev, Jury M., Meister, Jean-Jacques, Bershadsky, Alexander D., Verkhovsky, Alexander B.·2008·PLoS ONE
DOI 10.1371/journal.pone.0003234PMC PMC2535565
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Abstract

Dynamic actin network at the leading edge of the cell is linked to the extracellular matrix through focal adhesions (FAs), and at the same time it undergoes retrograde flow with different dynamics in two distinct zones: the lamellipodium (peripheral zone of fast flow), and the lamellum (zone of slow flow located between the lamellipodium and the cell body). Cell migration involves expansion of both the lamellipodium and the lamellum, as well as formation of new FAs, but it is largely unknown how the position of the boundary between the two flow zones is defined, and how FAs and actin flow mutually influence each other. We investigated dynamic relationship between focal adhesions and the boundary between the two flow zones in spreading cells. Nascent FAs first appeared in the lamellipodium. Within seconds after the formation of new FAs, the rate of actin flow decreased locally, and the lamellipodium/lamellum boundary advanced towards the new FAs. Blocking fast actin flow with cytochalasin D resulted in rapid dissolution of nascent FAs. In the absence of FAs (spreading on poly-L-lysine-coated surfaces) retrograde flow was uniform and the velocity transition was not observed. We conclude that formation of FAs depends on actin dynamics, and in its turn, affects the dynamics of actin flow by triggering transition from fast to slow flow. Extension of the cell edge thus proceeds through a cycle of lamellipodium protrusion, formation of new FAs, advance of the lamellum, and protrusion of the lamellipodium from the new base.

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  • Arp2/3‐dependent branched actin has a specific role in counterbalancing microtubule‐induced contractile tension, potentially through its coupling to nascent adhesions and the substrate.

    “Instead, these results point to a specific role for Arp2/3‐dependent branched actin in counterbalancing microtubule‐induced contractile tension, potentially through its coupling to nascent adhesions and the substrate [38, 39]”

    From Paclitaxel induces <scp>NM2</scp> ‐dependent cellular contraction through <scp>GEF</scp> ‐ <scp>H1</scp> dissociation from microtubules and <scp>RhoA</scp> / <scp>ROCK</scp> activation in cancer cells (2026) · cites this paper as [38]

    The Discussion (page unavailable in our copy) links lamellipodial actin flow to nascent adhesions and discusses a possible Arp2/3–vinculin connection to adhesion. It does not say that branched actin counterbalances microtubule-induced contractile tension; that part of the attributed claim is unsupported by this source. (LLM, conf=0.85)

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Authors
Alexandrova, A. Y., Arnold, K., Schaub, S., Vasiliev, J. M., Meister, J.-J., Bershadsky, A. D., & Verkhovsky, A. B.
Affiliations
  • Lomonosov Moscow State University (RU)
  • Weizmann Institute of Science (IL)
  • Centre National de la Recherche Scientifique (FR)
  • École Polytechnique Fédérale de Lausanne (CH)
Corresponding author
  • Bershadsky, Alexander D.
  • Verkhovsky, Alexander B.
ORCID
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2008-09-18
Volume
3
Issue
9
Pages
e3234
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0003234
PMC
PMC2535565
OpenAlex
W2146667247
Cited by
275
References
0
Citations by year
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FWCI
6.51
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • National University of Singapore (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • Figshare (submittedVersion, cc-by)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
Keywords
actin retrograde flow, focal adhesions, nascent adhesions, lamellipodia, cell migration, cell spreading, actin dynamics, cytochalasin D, extracellular matrix adhesion
MeSH terms
Actins, Animals, Cell Movement, Cytoskeleton, Extracellular Matrix, Melanoma, Experimental, Actin Cytoskeleton, Microscopy, Microscopy, Phase-Contrast, Polylysine, Cytochalasin D, 3T3 Cells, Focal Adhesions, Mice, Rats

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