Kinetics of Wnt-driven β-catenin stabilization revealed by quantitative and temporal imaging
Open accessAbstract
The Wnt/beta-catenin signal transduction pathway regulates a broad range of developmental processes. Aberrant activation of the Wnt pathway leads to cancer and degenerative diseases. beta-catenin is a key signaling molecule that is frequently used as a direct monitor of Wnt pathway activation. This paper describes a multi-parametric method for quantitative analysis of cellular beta-catenin protein levels in a rapid and high-throughput manner. The assay offers temporally resolved detection of Wnt-stimulated accumulation of beta-catenin, simultaneously detecting cell number, and it sheds light onto the kinetics of posttranslational stabilization of beta-catenin.
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Wet-lab data from just three studies, [105-107], have been reused by simulation studies.
“When comparing Fig 6 and S1 Fig, we find that the wet-lab data from just three studies, namely from [105–107], have been reused by simulation studies.”
From Relating simulation studies by provenance—Developing a family of Wnt signaling models (2021) · cites this paper as [106]
A second cluster forms around the simulation studies [45, 46, 51, 56, 57] and either includes the same wet-lab data from [105, 106], the Cell cycle [46] or ROS [51, 57].
“A second cluster forms around the simulation studies [45, 46, 51, 56, 57] and either includes the same wet-lab data from [105, 106], the Cell cycle [46] or ROS [51, 57].”
From Relating simulation studies by provenance—Developing a family of Wnt signaling models (2021) · cites this paper as [106]
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References 17 · Cited by 42
Research works printed but not linked: 16
- [1]Fuerer C Nusse R Ten Berge D 2008 Wnt signalling in development and disease. Max Delbruck Center for Molecular Medicine meeting on Wnt signaling in Development and Disease. EMBO Rep 9 134 138 18188179 PMC2246409
- [2]Polakis P 2007 The many ways of Wnt in cancer. Curr Opin Genet Dev 17 45 51 17208432
- [3]Logan CY Nusse R 2004 The Wnt signaling pathway in development and disease. Annu Rev Cell Dev Biol 20 781 810 15473860
- [4]Kim K Lu Z Hay ED 2002 Direct evidence for a role of beta-catenin/LEF-1 signaling pathway in induction of EMT. Cell Biol Int 26 463 476 12095232
- [5]Naik S Piwnica-Worms D 2007 Real-time imaging of beta-catenin dynamics in cells and living mice. Proc Natl Acad Sci U S A 104 17465 17470 17954915 PMC2077279
- [6]Kishida M Koyama S Kishida S Matsubara K Nakashima S 1999 Axin prevents Wnt-3a-induced accumulation of b-catenin. Oncogene 18 979 985 10023673
- [7]Smith PJ Wiltshire M Davies S Patterson LH Hoy T 1999 A novel cell permeant and far red-fluorescing DNA probe, DRAQ5™, for blood cell discrimination by flow cytometry. Journal of Immunological Methods 229 131 139 10556697
- [8]Yun M-S Kim S-E Jeon SH Lee J-S Choi K-Y 2005 Both ERK and Wnt/ß-catenin pathways are involved in Wnt3a-induced proliferation. Journal of Cell Science 118 313 322 15615777
- [9]Polakis P 2000 Wnt signaling and cancer. Genes Dev 14 1837 1851 10921899
- [10]Rubinfeld B Albert I Porfiri E Fiol C Munemitsu S 1996 Binding of GSK3beta to the APC-beta-catenin complex and regulation of complex assembly. Science 272 1023 1026 8638126
- [11]Klein PS Melton DA 1996 A molecular mechanism for the effect of lithium on development. Proc Natl Acad Sci U S A 93 8455 8459 8710892 PMC38692
- [12]Lee DH Goldberg AL 1996 Selective inhibitors of the proteasome-dependent and vacuolar pathways of protein degradation in Saccharomyces cerevisiae. J Biol Chem 271 27280 27284 8910302
- [13]Park CH Chang JY Hahm ER Park S Kim HK 2005 Quercetin, a potent inhibitor against beta-catenin/Tcf signaling in SW480 colon cancer cells. Biochem Biophys Res Commun 328 227 234 15670774
- [14]Emami KH Nguyen C Ma H Kim DH Jeong KW 2004 A small molecule inhibitor of beta-catenin/CREB-binding protein transcription. Proc Natl Acad Sci U S A 101 12682 12687 15314234 PMC515116
- [15]Lee E Salic A Kruger R Heinrich R Kirschner MW 2003 The roles of APC and Axin derived from experimental and theoretical analysis of the Wnt pathway. PLoS Biol 1 E10 14551908 PMC212691
- [16]Rubinfeld B Robbins P El-Gamil M Albert I Porfiri E 1997 Stabilization of beta-catenin by genetic defects in melanoma cell lines. Science 275 1790 1792 9065403
- Authors
- Hannoush, R. N.
- Affiliations
- Genentech (US)
- Corresponding author
- Hannoush, Rami N.
- ORCID
- Hannoush, Rami N.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2008-10-22
- Volume
- 3
- Issue
- 10
- Pages
- e3498
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC2570213
- OpenAlex
- W2032112650
- Cited by
- 42
- References
- 17
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 1 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 4 · 2017: 7 · 2016: 3 · 2015: 4 · 2014: 2 · 2013: 2 · 2012: 4
- FWCI
- 0.96
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Funders
- Genentech
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
- Keywords
- Wnt/β-catenin signaling, β-catenin stabilization, protein accumulation, quantitative imaging, temporal imaging, high-throughput assay
- MeSH terms
- Animals, Cell Line, Fibroblasts, Fluorescent Antibody Technique, Kinetics, Signal Transduction, Proteomics, Wnt Proteins, beta Catenin, Mice, Protein Stability
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