The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Temporally-Controlled Site-Specific Recombination in Zebrafish
Open accessAbstract
Conventional use of the site-specific recombinase Cre is a powerful technology in mouse, but almost absent in other vertebrate model organisms. In zebrafish, Cre-mediated recombination efficiency was previously very low. Here we show that using transposon-mediated transgenesis, Cre is in fact highly efficient in this organism. Furthermore, temporal control of recombination can be achieved by using the ligand-inducible CreER(T2). Site-specific recombination only occurs upon administration of the drug tamoxifen (TAM) or its active metabolite, 4-hydroxy-tamoxifen (4-OHT). Cre-mediated recombination is detectable already 4 or 2 hours after administration of TAM or 4-OHT, demonstrating fast recombination kinetics. In addition, low doses of TAM allow mosaic labeling of single cells. Combined, our results show that conditional Cre/lox will be a valuable tool for both, embryonic and adult zebrafish studies. Furthermore, single copy insertion transgenesis of Cre/lox constructs suggest a strategy suitable also for other organisms.
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Metzger, D., Clifford, J., Chiba, H., & Chambon, P. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.15.6991 · Cited by 570 · cited 2×
“The following enzymes were used: SalI and FseI for the pax2a promoter [23] and FseI and AscI for the CreER gene [12].”
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Mosimann, C., Kaufman, C. K., Li, P., Pugach, E. K., Tamplin, O. J., & Zon, L. I. (2011) · Development · doi:10.1242/dev.059345 · Cited by 416
Adult neurogenesis and brain regeneration in zebrafish
Kizil, C., Kaslin, J., Kroehne, V., & Brand, M. (2012) · Developmental Neurobiology · doi:10.1002/dneu.20918 · Cited by 349
Self-citationOptimized Gal4 genetics for permanent gene expression mapping in zebrafish
Distel, M., Wullimann, M. F., & Köster, R. W. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0903060106 · Cited by 289
Johnson, S. L., Nguyen, A. N., & Lister, J. A. (2011) · Developmental Biology · doi:10.1016/j.ydbio.2010.12.004 · Cited by 85
Metadata
- Authors
- Hans, S., Kaslin, J., Freudenreich, D., & Brand, M.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2009-02-27
- Volume
- 4
- Issue
- 2
- Pages
- e4640
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2093279832
- Cited by
- 198
- References
- 1
- FWCI
- 8.44
- Type
- journal article
- Language
- en
- Open access
- gold
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
