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Cited by 100Ion channel regulation and functionNeuroscience and Neuropharmacology ResearchNeural dynamics and brain function

Correlations in ion channel mRNA in rhythmically active neurons

Open access
Tobin, Anne-Elise, Cruz-Bermúdez, Nelson D., Marder, Eve, Schulz, David J.·2009·PLoS ONE
DOI 10.1371/journal.pone.0006742PMC PMC2727049
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Abstract

BACKGROUND: To what extent do identified neurons from different animals vary in their expression of ion channel genes? In neurons of the same type, is ion channel expression highly variable and/or is there any relationship between ion channel expression that is conserved? METHODOLOGY/PRINCIPAL FINDINGS: To address these questions we measured ion channel mRNA in large cells (LCs) of the crab cardiac ganglion. We cloned a calcium channel, caco, and a potassium channel, shaker. Using single-cell quantitative PCR, we measured levels of mRNA for these and 6 other different ion channels in cardiac ganglion LCs. Across the population of LCs we measured 3-9 fold ranges of mRNA levels, and we found correlations in the expression of many pairs of conductances CONCLUSIONS/SIGNIFICANCE: In previous measurements from the crab stomatogastric ganglion (STG), ion channel expression was variable, but many pairs of channels had correlated expression. However, each STG cell type had a unique combination of ion channel correlations. Our findings from the crab cardiac ganglion are similar, but the correlations in the LCs are different from those in STG neurons, supporting the idea that such correlations could be markers of cell identity or activity.

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  • In wild-type populations of animals with similar performance profiles, 2-7 fold variations in the mRNA expression of most cellular components are seen in identified neurons across individuals.

    “In wild-type populations of animals with similar performance profiles, 2-7 fold variations in the mRNA expression of most cellular components are seen in identified neurons across individuals [25-30]”

    From A feature and not a bug: Replication failures can lead to new biology (2026) · cites this paper as [30]

    The Introduction describes variable ion-channel mRNA levels in identified neurons despite similar activity, and the Results report variation across cardiac-ganglion cells. But the paper measures eight ion-channel transcripts, not most cellular components; its reported range is 3–9 fold, not 2–7 fold. The cited passages do not establish the wild-type qualifier. (LLM, conf=0.88)

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Authors
Tobin, A.-E., Cruz-Bermúdez, N. D., Marder, E., & Schulz, D. J.
Affiliations
  • Brandeis University (US)
  • University of Missouri (US)
Corresponding author
Tobin, Anne-Elise
ORCID
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2009-08-25
Volume
4
Issue
8
Pages
e6742
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0006742
PMC
PMC2727049
OpenAlex
W1966509643
Cited by
100
References
1
Citations by year
2026: 3 · 2024: 4 · 2023: 4 · 2022: 7 · 2021: 6 · 2020: 2 · 2019: 6 · 2018: 7 · 2017: 2 · 2016: 6 · 2015: 8 · 2014: 13 · 2013: 6 · 2012: 9
FWCI
3.25
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Brandeis Institutional Repository (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Science Foundation (0615160)
  • National Institutes of Health (MH 46742)
  • National Institute of Mental Health (MH 46742, R37MH046742, R01MH046742)
  • National Institute of Neurological Disorders and Stroke (F32NS059255, NS059255)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
Keywords
ion channel gene expression, correlated gene expression, cardiac ganglia, crabs, Shaker potassium channel, stomatogastric ganglion, cell identity, neuronal activity
MeSH terms
Animals, Base Sequence, Cloning, Molecular, Crustacea, Heart, Neurons, RNA, Messenger, Potassium Channels, Sodium Channels, DNA Primers, Reverse Transcriptase Polymerase Chain Reaction

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