Genetic composition of laboratory stocks of the self-fertilizing fish Kryptolebias marmoratus: a valuable resource for experimental research
Open accessAbstract
The hermaphroditic Mangrove Killifish, Kryptolebias marmoratus, is the world's only vertebrate that routinely self-fertilizes. As such, highly inbred and presumably isogenic "clonal" lineages of this androdioecious species have long been maintained in several laboratories and used in a wide variety of experiments that require genetically uniform vertebrate specimens. Here we conduct a genetic inventory of essentially all laboratory stocks of the Mangrove Killifish held worldwide. At 32 microsatellite loci, these stocks proved to show extensive interline differentiation as well as some intraline variation, much of which can be attributed to post-origin de novo mutations and/or to the segregation of polymorphisms from wild progenitors. Our genetic findings also document that many of the surveyed laboratory strains are not what they have been labeled, apparently due to the rather frequent mishandling or unintended mixing of various laboratory stocks over the years. Our genetic inventory should help to clarify much of this confusion about the clonal identities and genetic relationships of laboratory lines, and thereby help to rejuvenate interest in K. marmoratus as a reliable vertebrate model for experimental research that requires or can capitalize upon "clonal" replicate specimens.
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Lineages of K. marmoratus were independently perpetuated by self-fertilization, resulting in a purging of within-lineage genetic variation and creation of isogenic strains.
“Lineages were independently perpetuated by self-fertilization, resulting in a purging of within-lineage genetic variation and creation of isogenic strains (Tatarenkov et al., 2010).”
From Genomic and physiological mechanisms underlying skin plasticity during water to air transition in an amphibious fish (2020) · cites this paper as Tatarenkov et al., 2010
The Introduction says repeated self-fertilization promotes high genetic uniformity, and the Results (Intraline variation; page unavailable) identify 11 lines as truly isogenic. But the same Results report that specimens in the other 10 lines were not all genetically identical, so the claim overstates the extent to which self-fertilization eliminated within-lineage variation. (LLM, conf=0.88)
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References 91 · Cited by 89
Research works printed but not linked: 7
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- [23]McMillan CM 1979 The embryological development of Rivulus marmoratus. M.S. thesis. Charleston, South Carolina College of Charleston
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- Authors
- Tatarenkov, A., Ring, B. C., Elder, J. F., Bechler, D. L., & Avise, J. C.
- Affiliations
- University of California, Irvine (US)
- Valdosta State University (US)
- Corresponding author
- Tatarenkov, Andrey
- Ring, Brian C.
- ORCID
- Tatarenkov, Andrey
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2010-09-22
- Volume
- 5
- Issue
- 9
- Pages
- e12863
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC2943930
- OpenAlex
- W2019531106
- Cited by
- 89
- References
- 91
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 4 · 2023: 4 · 2022: 4 · 2021: 9 · 2020: 8 · 2019: 10 · 2018: 5 · 2017: 9 · 2016: 5 · 2015: 5 · 2014: 4 · 2012: 9
- FWCI
- 2.52
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- eScholarship (California Digital Library) (submittedVersion, cc-by)
- Figshare (submittedVersion, cc-by)
- Funders
- Valdosta State University
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R15HD060017)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Animal Behavior and Reproduction
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Fish Biology and Ecology Studies
- Keywords
- Kryptolebias marmoratus, mangrove killifish, self-fertilization, androdioecy, clonal lineages, microsatellite markers, inbreeding, de novo mutations, vertebrate model organisms
- MeSH terms
- Animals, Killifishes, Phylogeny, Research, Microsatellite Repeats, Self-Fertilization
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