An in vivo polymicrobial biofilm wound infection model to study interspecies interactions
Open accessAbstract
Chronic wound infections are typically polymicrobial; however, most in vivo studies have focused on monospecies infections. This project was designed to develop an in vivo, polymicrobial, biofilm-related, infected wound model in order to study multispecies biofilm dynamics and in relation to wound chronicity. Multispecies biofilms consisting of both Gram negative and Gram positive strains, as well as aerobes and anaerobes, were grown in vitro and then transplanted onto the wounds of mice. These in vitro-to-in vivo multi-species biofilm transplants generated polymicrobial wound infections, which remained heterogeneous with four bacterial species throughout the experiment. We observed that wounded mice given multispecies biofilm infections displayed a wound healing impairment over mice infected with a single-species of bacteria. In addition, the bacteria in the polymicrobial wound infections displayed increased antimicrobial tolerance in comparison to those in single species infections. These data suggest that synergistic interactions between different bacterial species in wounds may contribute to healing delays and/or antibiotic tolerance.
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In vitro-generated mixed species biofilms have been shown to delay healing when placed in a murine wound model.
“in vitro-generated mixed species biofilms have been shown to delay healing when placed in a murine wound model [42]”
From Interactions of Methicillin Resistant Staphylococcus aureus USA300 and Pseudomonas aeruginosa in Polymicrobial Wound Infection (2013) · cites this paper as [42]
Few in vivo studies have focused on the synergistic effects of S. aureus and P. aeruginosa within the wound environment.
“However, despite the exceedingly high frequency of S. aureus and P. aeruginosa in chronic and acute wounds, few in vivo studies have focused on the synergistic effects of these prevalent bacteria within the wound environment [39], [42].”
From Interactions of Methicillin Resistant Staphylococcus aureus USA300 and Pseudomonas aeruginosa in Polymicrobial Wound Infection (2013) · cites this paper as [42]
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References 53 · Cited by 372
Research works printed but not linked: 1
- [2]Center for Disease Control 2002 National estimates and general information on diabetes in the U.S.. Department of Health and Human Services, Centers for Disease Control and Prevention (Eds.) Atlanta, GA Revised edition in:Center U.S
- Authors
- Dalton, T., Dowd, S. E., Wolcott, R. D., Sun, Y., Watters, C., Griswold, J. A., & Rumbaugh, K. P.
- Affiliations
- Texas Tech University (US)
- Corresponding author
- Rumbaugh, Kendra P.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2011-11-04
- Volume
- 6
- Issue
- 11
- Pages
- e27317
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3208625
- OpenAlex
- W2001963171
- Cited by
- 372
- References
- 53
- Citations by year
- 2026: 19 · 2025: 12 · 2024: 28 · 2023: 38 · 2022: 36 · 2021: 33 · 2020: 48 · 2019: 22 · 2018: 29 · 2017: 17 · 2016: 26 · 2015: 18 · 2014: 24 · 2013: 10 · 2012: 11
- FWCI
- 5.81
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- American Diabetes Association
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Health Sciences › Medicine › Surgery › Orthopedic Infections and Treatments
- Keywords
- polymicrobial biofilms, chronic wound infection, interspecies interactions, delayed wound healing, antimicrobial tolerance, antibiotic resistance, Gram-negative bacteria, Gram-positive bacteria
- MeSH terms
- Animals, Anti-Bacterial Agents, Bacteria, Bacterial Infections, Chronic Disease, DNA, Bacterial, Species Specificity, Wound Healing, Wound Infection, Biofilms, Biodiversity, Mice, Microbial Interactions, Real-Time Polymerase Chain Reaction
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