Fgf signaling is required for photoreceptor maintenance in the adult zebrafish retina
Open accessAbstract
Fibroblast growth factors (Fgf) are secreted signaling molecules that have mitogenic, patterning, neurotrophic and angiogenic properties. Their importance during embryonic development in patterning and morphogenesis of the vertebrate eye is well known, but less is known about the role of Fgfs in the adult vertebrate retina. To address Fgf function in adult retina, we determined the spatial distribution of components of the Fgf signaling pathway in the adult zebrafish retina. We detected differential expression of Fgf receptors, ligands and downstream Fgf targets within specific retinal layers. Furthermore, we blocked Fgf signaling in the retina, by expressing a dominant negative variant of Fgf receptor 1 conditionally in transgenic animals. After blocking Fgf signaling we observe a fast and progressive photoreceptor degeneration and disorganization of retinal tissue, coupled with cell death in the outer nuclear layer. Following the degeneration of photoreceptors, a profound regeneration response is triggered that starts with proliferation in the inner nuclear layer. Ultimately, rod and cone photoreceptors are regenerated completely. Our study reveals the requirement of Fgf signaling to maintain photoreceptors and for proliferation during regeneration in the adult zebrafish retina.
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Fgfs and their receptors are expressed in the inner nuclear layer of the zebrafish retina.
“Fgfs and their receptors are expressed in the INL of the zebrafish retina (Hochmann et al., 2012);”
From Retinal injury, growth factors, and cytokines converge on β-catenin and pStat3 signaling to stimulate retina regeneration (2014) · cites this paper as Hochmann et al., 2012
Conditional expression of dnFgfr1 suppresses Müller glia proliferation in the injured retina.
“We found that conditional expression of dnFgfr1 suppressed MG proliferation in the injured retina (Figures 2G and 2H), which is consistent with that found when photoreceptors are damaged (Hochmann et al., 2012).”
From Retinal injury, growth factors, and cytokines converge on β-catenin and pStat3 signaling to stimulate retina regeneration (2014) · cites this paper as Hochmann et al., 2012
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References 63 · Cited by 62
- Authors
- Hochmann, S., Kaslin, J., Hans, S., Weber, A., Machate, A., Geffarth, M., Funk, R. H. W., & Brand, M.
- Affiliations
- Center for Regenerative Therapies Dresden (DE)
- Technische Universität Dresden (DE)
- Corresponding author
- Brand, Michael
- ORCID
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2012-01-26
- Volume
- 7
- Issue
- 1
- Pages
- e30365
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3266925
- OpenAlex
- W1987520439
- Cited by
- 62
- References
- 63
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 3 · 2023: 1 · 2022: 5 · 2021: 6 · 2020: 9 · 2019: 4 · 2018: 4 · 2017: 4 · 2016: 4 · 2015: 1 · 2014: 6 · 2013: 8 · 2012: 3
- FWCI
- 2.32
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Monash University Research Portal (Monash University) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Funders
- European Commission
- Deutsche Forschungsgemeinschaft (SFB 655)
- Zentrum für Regenerative Therapien Dresden
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
- Keywords
- FGF signaling, photoreceptor degeneration, retinal regeneration, zebrafish retina, FGF receptor 1, dominant negative receptor, rod and cone photoreceptors, adult retina
- MeSH terms
- Age Factors, Animals, Cell Survival, Fibroblast Growth Factors, Retina, Zebrafish, Signal Transduction, Cell Death, Receptors, Fibroblast Growth Factor, Gene Expression Regulation, Developmental, Photoreceptor Cells, Vertebrate, Animals, Genetically Modified, Receptor, Fibroblast Growth Factor, Type 1
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