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Cited by 337Marine and coastal ecosystemsDiatoms and Algae ResearchAquatic Ecosystems and Phytoplankton Dynamics

The Transcriptome and Proteome of the Diatom Thalassiosira pseudonana Reveal a Diverse Phosphorus Stress Response

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Dyhrman, Sonya T., Jenkins, Bethany D., Rynearson, Tatiana A., Saito, Mak A., Mercier, Melissa L., Alexander, Harriet, Whitney, LeAnn P., Drzewianowski, Andrea +5 more·2012·PLoS ONE
DOI 10.1371/journal.pone.0033768PMC PMC3315573
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Abstract

Phosphorus (P) is a critical driver of phytoplankton growth and ecosystem function in the ocean. Diatoms are an abundant class of marine phytoplankton that are responsible for significant amounts of primary production. With the control they exert on the oceanic carbon cycle, there have been a number of studies focused on how diatoms respond to limiting macro and micronutrients such as iron and nitrogen. However, diatom physiological responses to P deficiency are poorly understood. Here, we couple deep sequencing of transcript tags and quantitative proteomics to analyze the diatom Thalassiosira pseudonana grown under P-replete and P-deficient conditions. A total of 318 transcripts were differentially regulated with a false discovery rate of <0.05, and a total of 136 proteins were differentially abundant (p<0.05). Significant changes in the abundance of transcripts and proteins were observed and coordinated for multiple biochemical pathways, including glycolysis and translation. Patterns in transcript and protein abundance were also linked to physiological changes in cellular P distributions, and enzyme activities. These data demonstrate that diatom P deficiency results in changes in cellular P allocation through polyphosphate production, increased P transport, a switch to utilization of dissolved organic P through increased production of metalloenzymes, and a remodeling of the cell surface through production of sulfolipids. Together, these findings reveal that T. pseudonana has evolved a sophisticated response to P deficiency involving multiple biochemical strategies that are likely critical to its ability to respond to variations in environmental P availability.

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  • In 2012, Dyhrman et al. identified cell surface-associated APase, suggesting that Pi hydrolysis and Pi uptake may be tightly coupled.

    “In 2012, Dyhrman et al. [34] identified cell surface–associated APase, suggesting that Pi hydrolysis and Pi uptake may be “tightly coupled.””

    From A Review of Phosphate Mineral Nucleation in Biology and Geobiology (2013) · cites this paper as [34]

  • Dyhrman et al. identified polyP polymerase regulation, with cellular P condensing to polyP.

    “They also identified polyP polymerase regulation, with cellular P condensing to polyP”

    From A Review of Phosphate Mineral Nucleation in Biology and Geobiology (2013) · cites this paper as [34]

  • Alkaline phosphatases are theorized to cleave inorganic phosphate from polyphosphate in diatoms.

    “APases cleave Pi from ester phosphates at neutral to basic pH [33] and are theorized to cleave Pi from polyP in diatoms [34].”

    From A Review of Phosphate Mineral Nucleation in Biology and Geobiology (2013) · cites this paper as [34]

    The Results and Discussion section describes alkaline phosphatases acting on phosphomonoesters and separately discusses polyphosphate accumulation and cycling. Neither that section nor the Conclusions says that alkaline phosphatases are theorized to release inorganic phosphate from polyphosphate; searches for that proposed link found no passage addressing it. (LLM, conf=0.75)

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Authors
Dyhrman, S. T., Jenkins, B. D., Rynearson, T. A., Saito, M. A., Mercier, M. L., Alexander, H., Whitney, L. P., Drzewianowski, A., Bulygin, V. V., Bertrand, E. M., Wu, Z., Benitez-Nelson, C., & Heithoff, A.
Affiliations
  • Woods Hole Oceanographic Institution (US)
  • University of Rhode Island (US)
  • Brown University (US)
Corresponding author
  • Dyhrman, Sonya T.
  • Jenkins, Bethany D.
  • Rynearson, Tatiana A.
  • Saito, Mak A.
  • Mercier, Melissa L.
  • Alexander, Harriet
  • Whitney, LeAnn P.
  • Drzewianowski, Andrea
  • Bulygin, Vladimir V.
  • Bertrand, Erin M.
  • Wu, Zhijin
  • Heithoff, Abigail
ORCID
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2012-03-29
Volume
7
Issue
3
Pages
e33768
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0033768
PMC
PMC3315573
OpenAlex
W2075935267
Cited by
337
References
76
Citations by year
2026: 18 · 2025: 17 · 2024: 17 · 2023: 32 · 2022: 26 · 2021: 20 · 2020: 26 · 2019: 33 · 2018: 26 · 2017: 29 · 2016: 28 · 2015: 22 · 2014: 22 · 2013: 12 · 2012: 7
FWCI
14.88
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Figshare (submittedVersion, cc-by)
  • Journal of Media Literacy Education (submittedVersion, cc-by-nc)
  • Scholar Commons (University of South Carolina) (submittedVersion, cc-by)
  • Open Access Server of the Woods Hole Scientific Community (Woods Hole Scientific Community) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Science Foundation (0752291, 0526800, 0723667, 0549794, 0554548, 1004057)
  • U.S. Department of Energy
  • Gordon and Betty Moore Foundation
  • Joint Genome Institute
  • Office of Experimental Program to Stimulate Competitive Research (0554548, 1004057)
Topics
  • Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal ecosystems
  • Physical Sciences › Materials Science › Biomaterials › Diatoms and Algae Research
  • Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
Keywords
Thalassiosira pseudonana, diatoms, phosphorus deficiency, transcriptome, proteome, phosphorus transport, dissolved organic phosphorus, metalloenzymes, sulfolipids, glycolysis, translation (biology), nutrient limitation
MeSH terms
Biological Transport, Glycolysis, Phosphorus, Stress, Physiological, Protein Biosynthesis, Diatoms, Proteome, Transcriptome

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