The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
Identifying mRNA, MicroRNA and Protein Profiles of Melanoma Exosomes
Open accessAbstract
BACKGROUND: Exosomes are small membranous vesicles secreted into body fluids by multiple cell types, including tumor cells, and in various disease conditions. Tumor exosomes contain intact and functional mRNAs, small RNAs (including miRNAs), and proteins that can alter the cellular environment to favor tumor growth. Molecular profiling may increase our understanding of the role of exosomes in melanoma progression and may lead to discovery of useful biomarkers. METHODOLOGY/PRINCIPAL FINDINGS: In the present study, we used mRNA array profiling to identify thousands of exosomal mRNAs associated with melanoma progression and metastasis. Similarly, miRNA array profiling identified specific miRNAs, such as hsa-miR-31, -185, and -34b, involved in melanoma invasion. We also used proteomic analysis and discovered differentially expressed melanoma exosomal proteins, including HAPLN1, GRP78, syntenin-1, annexin A1, and annexin A2. Importantly, normal melanocytes acquired invasion ability through molecules transported in melanoma cell-derived exosomes. CONCLUSIONS/SIGNIFICANCE: Our results indicate that melanoma-derived exosomes have unique gene expression signatures, miRNA and proteomics profiles compared to exosomes from normal melanocytes. To the best of our knowledge, this is the first in-depth screening of the whole transcriptome/miRNome/proteome expression in melanoma exosomes. These results provide a starting point for future more in-depth studies of tumor-derived melanoma exosomes, which will aid our understanding of melanoma biogenesis and new drug-targets that may be translated into clinical applications, or as non-invasive biomarkers for melanoma.
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1 retracted
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Self-citationExosomes: proteomic insights and diagnostic potential
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Exosomes Released by Melanoma Cells Prepare Sentinel Lymph Nodes for Tumor Metastasis
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Cited by
Methods of isolating extracellular vesicles impact down-stream analyses of their cargoes
Taylor, D. D., & Shah, S. (2015) · Methods · doi:10.1016/j.ymeth.2015.02.019 · Cited by 629
Self-citationUltra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen
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Exosomes as divine messengers: are they the Hermes of modern molecular oncology?
Braicu, C., Tomuleasa, C., Monroig, P., Cucuianu, A., Berindan-Neagoe, I., & Calin, G. A. (2015) · Cell Death & Differentiation · doi:10.1038/cdd.2014.130 · Cited by 297
Annexin-Phospholipid Interactions. Functional Implications
Lizarbe, M., Barrasa, J., Olmo, N., Gavilanes, F., & Turnay, J. (2013) · International Journal of Molecular Sciences · doi:10.3390/ijms14022652 · Cited by 234
Metadata
- Authors
- Xiao, D., Ohlendorf, J., Chen, Y., Taylor, D. D., Rai, S. N., Waigel, S., Zacharias, W., Hao, H., & McMasters, K. M.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2012-10-09
- Volume
- 7
- Issue
- 10
- Pages
- e46874
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2065723650
- Cited by
- 277
- References
- 47
- FWCI
- 8.36
- Type
- journal article
- Language
- en
- Open access
- gold
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