CoChat
Sign Up
Cited by 135Viral Infections and Immunology ResearchViral Infections and Outbreaks ResearchInfectious Encephalopathies and Encephalitis

The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.

Circulation of Coxsackievirus A10 and A6 in Hand-Foot-Mouth Disease in China, 2009–2011

Open access
Lu, Qing-Bin, Zhang, Xiao-Ai, Wo, Ying, Xu, Hong-Mei, Li, Xiu-Jun, Wang, Xian-Jun, Ding, Shu-Jun, Chen, Xiao-Dan, He, Cui, Liu, Li-Juan, Li, Hao, Yang, Hong, Li, Ting-Yu, Liu, Wei, Cao, Wu-Chun·2012·PLoS ONE
DOI 10.1371/journal.pone.0052073PMC PMC3525556
Open this paper in CoChat — free accountOpen PDF

Abstract

Coxsackieviruses A10 (CV-A10) and A6 (CV-A6) have been associated with increasingly occurred sporadic hand-foot-mouth disease (HFMD) cases and outbreak events globally. However, our understanding of epidemiological and genetic characteristics of these new agents remains far from complete. This study was to explore the circulation of CV-A10 and CV-A6 in HFMD and their genetic characteristics in China. A hospital based surveillance was performed in three heavily inflicted regions with HFMD from March 2009 to August 2011. Feces samples were collected from children with clinical diagnosis of HFMD. The detection and genotyping of enteroviruses was performed by real-time PCR and sequencing of 5'UTR/VP1 regions. Phylogenetic analysis and selection pressure were performed based on the VP1 sequences. Logistic regression model was used to identify the effect of predominant enterovirus serotypes in causing severe HFMD. The results showed 92.0% of 1748 feces samples were detected positive for enterovirus, with the most frequently presented serotypes as EV-71 (944, 54.0%) and CV-A16 (451, 25.8%). CV-A10 and CV-A6 were detected as a sole pathogen in 82 (4.7%) and 44 (2.5%) cases, respectively. Infection with CV-A10 and EV-71 were independently associated with high risk of severe HFMD (OR = 2.66, 95% CI: 1.40-5.06; OR = 4.81, 95% CI: 3.07-7.53), when adjusted for age and sex. Phylogenetic analysis revealed that distinct geographic and temporal origins correlated with the gene clusters based on VP1 sequences. An overall ω value of the VP1 was 0.046 for CV-A10 and 0.047 for CV-A6, and no positively selected site was detected in VP1 of both CV-A10 and CV-A6, indicating that purifying selection shaped the evolution of CV-A10 and CV-A6. Our study demonstrates variety of enterovirus genotypes as viral pathogens in causing HFMD in China. CV-A10 and CV-A6 were co-circulating together with EV-71 and CV-A16 in recent years. CV-A10 infection might also be independently associated with severe HFMD.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1371/journal.pone.0052073

Cites

29 of 29 references verified0 citation errors · 0 not checkable

Statistics

Figures

Figure 1 The enterovirus type identified in hand-foot-mouth diseases patients during Mar 2009 to Aug 2011, China. EV, enterovirus; CV, coxsackievirus; ECV, echovirus.
Figure 1 The enterovirus type identified in hand-foot-mouth diseases patients during Mar 2009 to Aug 2011, China. EV, enterovirus; CV, coxsackievirus; ECV, echovirus.CC BY · PubMed Central
Figure 2 The detailed distribution of enterovirus types in each province. EV, enterovirus; CV, coxsackievirus; ECV, echovirus.
Figure 2 The detailed distribution of enterovirus types in each province. EV, enterovirus; CV, coxsackievirus; ECV, echovirus.CC BY · PubMed Central
Figure 3 The temporal distribution of HEV types in hand-foot-mouth diseases patients during March 2009 to August 2011, China.
Figure 3 The temporal distribution of HEV types in hand-foot-mouth diseases patients during March 2009 to August 2011, China.CC BY · PubMed Central
Figure 4 Phylogenetic trees were constructed from the VP1 nucleotide sequences of CV-A10 using neighbor-joining method with 1000 bootstrap by CLC genomics workbench. The tree was based on the 379 bp VP1 nucleotide sequences of CV-A10 (nt 2630–3008, responding to AY421767). The strains labeled with green dot were obtained in our study.
Figure 4 Phylogenetic trees were constructed from the VP1 nucleotide sequences of CV-A10 using neighbor-joining method with 1000 bootstrap by CLC genomics workbench. The tree was based on the 379 bp VP1 nucleotide sequences of CV-A10 (nt 2630–3008, responding to AY421767). The strains labeled with green dot were obtained in our study.CC BY · PubMed Central
Figure 5 Phylogenetic trees were constructed from the VP1 nucleotide sequences of CV-A6 using neighbor-joining method with 1000 bootstrap by CLC genomics workbench. The tree was based on the 552 bp VP1 nucleotide sequences of CV-A6 (nt 2441–2992, responding to AY421764). The strains labeled with green dot were obtained in our study.
Figure 5 Phylogenetic trees were constructed from the VP1 nucleotide sequences of CV-A6 using neighbor-joining method with 1000 bootstrap by CLC genomics workbench. The tree was based on the 552 bp VP1 nucleotide sequences of CV-A6 (nt 2441–2992, responding to AY421764). The strains labeled with green dot were obtained in our study.CC BY · PubMed Central

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 29 references

Research works printed but not linked: 2

  1. H Yang, 2010, The genetic characterization of VP1 region of coxsackie virus A10 isolated from hand, foot and mouth disease cases in Shandong Province of China, Chin J Infect Dis, 28, 385
  2. Y-Q Wang, 2011, Molecular characterization of hand, foot and mouth disease related non-EV71 non-CoxA16 enterovirus in Beijing, Int J Virol, 18, 75

Cited by

Sorted by citations

See all 135

Related

Metadata

Authors
Lu, Q.-B., Zhang, X.-A., Wo, Y., Xu, H.-M., Li, X.-J., Wang, X.-J., Ding, S.-J., Chen, X.-D., He, C., Liu, L.-J., Li, H., Yang, H., Li, T.-Y., Liu, W., & Cao, W.-C.
Affiliations
  • Shandong University (CN)
  • Beijing Institute of Microbiology and Epidemiology (CN)
Corresponding author
  • Liu, Wei
  • Cao, Wu-Chun
ORCID
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2012-12-18
Volume
7
Issue
12
Pages
e52073
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0052073
PMC
PMC3525556
OpenAlex
W1984450481
Cited by
135
References
29
Citations by year
2026: 2 · 2025: 4 · 2024: 8 · 2023: 5 · 2022: 9 · 2021: 6 · 2020: 3 · 2019: 14 · 2018: 14 · 2017: 10 · 2016: 14 · 2015: 23 · 2014: 17 · 2013: 6
FWCI
10.29
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
National Science Fund for Distinguished Young Scholars (30725032)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Viral Infections and Immunology Research
  • Health Sciences › Medicine › Infectious Diseases › Viral Infections and Outbreaks Research
  • Health Sciences › Medicine › Infectious Diseases › Infectious Encephalopathies and Encephalitis
Keywords
hand, foot and mouth disease, coxsackievirus A10, coxsackievirus A6, enterovirus genotypes, phylogenetic analysis, purifying selection, China, hospital surveillance, 5' untranslated region
MeSH terms
China, Coxsackievirus Infections, Disease Outbreaks, Enterovirus, Female, Genotype, Hand, Foot and Mouth Disease, Humans, Male, Molecular Sequence Data, Phylogeny, Serotyping, Viral Proteins, Sentinel Surveillance, History, 21st Century

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review