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Cited by 66Tissue Engineering and Regenerative MedicinePolymer Surface Interaction Studies3D Printing in Biomedical Research

The Effect of Heparin-VEGF Multilayer on the Biocompatibility of Decellularized Aortic Valve with Platelet and Endothelial Progenitor Cells

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Ye, Xiaofeng, Wang, Haozhe, Zhou, Jingxin, Li, Haiqing, Liu, Jun, Wang, Zhe, Chen, Anqing, Zhao, Qiang·2013·PLoS ONE
DOI 10.1371/journal.pone.0054622PMC PMC3554718
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Abstract

The application of polyelectrolyte multilayer films is a new, versatile approach to surface modification of decellularized tissue, which has the potential to greatly enhance the functionality of engineered tissue constructs derived from decellularized organs. In the present study, we test the hypothesis that Heparin- vascular endothelial growth factor (VEGF) multilayer film can not only act as an antithrombotic coating reagent, but also induce proliferation of endothelial progenitor cells (EPCs) on the decellularized aortic heart valve. SEM demonstrated the adhesion and geometric deformation of platelets. The quantitative assay of platelet activation was determined by measuring the production of soluble P-selectin. Binding and subsequent release of heparin and VEGF from valve leaflets were assessed qualitatively by laser confocal scanning microscopy and quantitatively by ELISA methods. Human blood derived EPCs were cultured and the adhesion and growth of EPCs on the surface modified valvular scaffolds were assessed. The results showed that Heparin-VEGF multilayer film improved decellularized valve haemocompatibility with respect to a substantial reduction of platelet adhesion. Release of VEGF from the decellularized heart valve leaflets at physiological conditions was sustained over 5 days. In vitro biological tests demonstrated that EPCs achieved better adhesion, proliferation and migration on the coatings with Heparin-VEGF multilayer film. Combined, these results indicate that Heparin-VEGF multilayer film could be used to cover the decellularized porcine aortic valve to decrease platelet adhesion while exhibiting excellent EPCs biocompatibility.

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  • Surface-immobilized heparin ameliorates hemocompatibility of coated surfaces by interacting with VEGFRs on ECs and preventing platelet adhesion.

    “Surface-immobilized heparin has been applied during preparation of different biomaterials to ameliorate the hemocompatibility of coated surfaces by interacting with vascular endothelial growth factor receptors (VEGFRs) on ECs and preventing platelet adhesion [225,226].”

    From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [226]

    The Abstract and Discussion report that a heparin–VEGF coating improved hemocompatibility of decellularized aortic valves by reducing platelet adhesion. The Discussion links VEGFR2 activation to progenitor cells contacting the coating’s VEGF layer, not to heparin interacting with VEGFRs. The study therefore does not support that proposed mechanism for surface-immobilized heparin alone. (LLM, conf=0.88)

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Research works printed but not linked: 28

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Cited by

Sorted by citations

Authors
Ye, X., Wang, H., Zhou, J., Li, H., Liu, J., Wang, Z., Chen, A., & Zhao, Q.
Affiliations
Shanghai Jiao Tong University (CN)
Corresponding author
Zhao, Qiang
ORCID
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2013-01-24
Volume
8
Issue
1
Pages
e54622
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0054622
PMC
PMC3554718
OpenAlex
W1970437304
Cited by
66
References
0
Citations by year
2026: 1 · 2025: 1 · 2024: 4 · 2023: 4 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 8 · 2018: 8 · 2017: 7 · 2016: 8 · 2015: 11 · 2014: 6 · 2013: 2
FWCI
8.88
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China (81000096)
  • Science and Technology Commission of Shanghai Municipality
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Keywords
polyelectrolyte multilayer coating, hemocompatibility, platelet adhesion, endothelial progenitor cells, antithrombotic coatings, VEGF release, P-selectin, tissue engineering scaffolds
MeSH terms
Aortic Valve, Biocompatible Materials, Blood Platelets, Cell Adhesion, Cells, Cultured, Endothelium, Vascular, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Heparin, Humans, Microscopy, Electron, Scanning, Stem Cells, Platelet Activation, Vascular Endothelial Growth Factor A, Cell Proliferation

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