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Cited by 40Light effects on plantsPhotosynthetic Processes and MechanismsPlant Molecular Biology Research

Phosphoinositides Play Differential Roles in Regulating Phototropin1- and Phototropin2-Mediated Chloroplast Movements in Arabidopsis

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Aggarwal, Chhavi, Łabuz, Justyna, Gabryś, Halina·2013·PLoS ONE
DOI 10.1371/journal.pone.0055393PMC PMC3566141
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Abstract

Phototropins are UVA/blue-light receptors involved in controlling the light-dependent physiological responses which serve to optimize the photosynthetic activity of plants and promote growth. The phototropin-induced phosphoinositide (PI) metabolism has been shown to be essential for stomatal opening and phototropism. However, the role of PIs in phototropin-induced chloroplast movements remains poorly understood. The aim of this work is to determine which PI species are involved in the control of chloroplast movements in Arabidopsis and the nature of their involvement. We present the effects of the inactivation of phospholipase C (PLC), PI3-kinase (PI3K) and PI4-kinase (PI4K) on chloroplast relocations in Arabidopsis. The inhibition of the phosphatidylinositol 4,5-bisphospahte [PI(4,5)P2]-PLC pathway, using neomycin and U73122, suppressed the phot2-mediated chloroplast accumulation and avoidance responses, without affecting movement responses controlled by phot1. On the other hand, PI3K and PI4K activities are more restricted to phot1- and phot2-induced weak-light responses. The inactivation of PI3K and PI4K by wortmannin and LY294002 severely affected the weak blue-light-activated accumulation response but had little effect on the strong blue-light-activated avoidance response. The inhibitory effect observed with PI metabolism inhibitors is, at least partly, due to a disturbance in Ca(2+) ((c)) signaling. Using the transgenic aequorin system, we show that the application of these inhibitors suppresses the blue-light-induced transient Ca(2+) ((c)) rise. These results demonstrate the importance of PIs in chloroplast movements, with the PI(4,5)P2-PLC pathway involved in phot2 signaling while PI3K and PI4K are required for the phot1- and phot2-induced accumulation response. Our results suggest that these PIs modulate cytosolic Ca(2+) signaling during movements.

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  • The PI(4,5)P2-PLC pathway is involved in light-avoidance movement of PHOT2 signaling, and PI3K and PI4K are required for the accumulation response induced by PHOT1 and PHOT2.

    “This result suggests that the PI (4,5) P2-PLC pathway is involved in light-avoidance movement of PHOT2 signaling and that PI3K and PI4K are required for the accumulation response induced by PHOT1 and PHOT2.”

    From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Aggarwal et al., 2013a, b

    The Introduction and Discussion link the PI(4,5)P2–PLC pathway to phot2-mediated avoidance and link PI3K and PI4K to phot1- and phot2-mediated accumulation. The Results report that kinase inhibitors severely reduced accumulation responses, but some responses remained; thus, calling both kinases “required” is stronger than the evidence establishes. Page numbers are not available in our copy. (LLM, conf=0.86)

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  • Inactivation of PI3K and PI4K with wortmannin and LY294002 severely affected the cumulative responses activated by weak blue light irradiation but had little effect on avoidance responses activated by strong blue light.

    “Inactivation of PI3K and PI4K with wortmannin and LY294002 severely affected the cumulative responses activated by weak blue light irradiation, but had little effect on avoidance responses activated by strong blue light.”

    From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Aggarwal et al., 2013a, b

  • Phosphoinositides regulate cytosolic Ca2+ signaling during chloroplast movement and ultimately regulate chloroplast light-gathering movement through chloroplast actin filaments.

    “These phosphoinositides regulate cytosolic Ca2+ signaling during chloroplast movement (Aggarwal et al., 2013a, b) (Fig. 2) and ultimately regulate the chloroplast light-gathering movement through chloroplast actin filaments.”

    From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Aggarwal et al., 2013a, b

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Authors
Aggarwal, C., Łabuz, J., & Gabryś, H.
Affiliations
Jagiellonian University (PL)
Corresponding author
Gabryś, Halina
ORCID
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2013-02-06
Volume
8
Issue
2
Pages
e55393
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0055393
PMC
PMC3566141
OpenAlex
W2005167935
Cited by
40
References
2
Citations by year
2026: 1 · 2025: 3 · 2023: 4 · 2022: 1 · 2020: 2 · 2019: 2 · 2018: 2 · 2017: 4 · 2016: 3 · 2015: 7 · 2014: 7 · 2013: 4
FWCI
8.45
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Homo Politicus (Academy of Humanities and Economics in Lodz) (submittedVersion, cc-by-nc-nd)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
Funders
European Commission (215174, POIG.02.01.00-12-064/08)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
Keywords
phototropin, chloroplast movement, phosphoinositides, phospholipase C, PI3-kinase, phosphatidylinositol 4,5-bisphosphate, calcium signaling, blue light signaling, Arabidopsis thaliana
MeSH terms
Calcium, Chloroplasts, Cytosol, Phosphatidylinositols, Phospholipases, Signal Transduction, Arabidopsis, Plant Leaves, Phototropism, Phosphatidylinositol 3-Kinases, 1-Phosphatidylinositol 4-Kinase, Arabidopsis Proteins, Phototropins

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