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Cited by 13Developmental Biology and Gene RegulationCongenital limb and hand anomaliesOsteoarthritis Treatment and Mechanisms

Misexpression of pknox2 in mouse limb bud mesenchyme perturbs zeugopod development and deltoid crest formation

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Zhou, Wenrong, Zhu, Huang, Zhao, Jianzhi, Li, Hanjun, Wan, Yong, Cao, Jingjing, Zhao, Haixia, Yu, Jian +8 more·2013·PLoS ONE
DOI 10.1371/journal.pone.0064237PMC PMC3661445
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Abstract

The TALE (Three Amino acid Loop Extension) family consisting of Meis, Pbx and Pknox proteins is a group of transcriptional co-factors with atypical homeodomains that play pivotal roles in limb development. Compared to the in-depth investigations of Meis and Pbx protein functions, the role of Pknox2 in limb development remains unclear. Here, we showed that Pknox2 was mainly expressed in the zeugopod domain of the murine limb at E10.5 and E11.5. Misexpression of Pknox2 in the limb bud mesenchyme of transgenic mice led to deformities in the zeugopod and forelimb stylopod deltoid crest, but left the autopod and other stylopod skeletons largely intact. These malformations in zeugopod skeletons were recapitulated in mice overexpressing Pknox2 in osteochondroprogenitor cells. Molecular and cellular analyses indicated that the misexpression of Pknox2 in limb bud mesenchyme perturbed the Hox10-11 gene expression profiles, decreased Col2 expression and Bmp/Smad signaling activity in the limb. These results indicated that Pknox2 misexpression affected mesenchymal condensation and early chondrogenic differentiation in the zeugopod skeletons of transgenic embryos, suggesting Pknox2 as a potential regulator of zeugopod and deltoid crest formation.

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  • PRRX1 and PKNOX2 have roles in mesenchyme development.

    “both of which have roles in mesenchyme development (56,57)”

    From SOX9 reprograms endothelial cells by altering the chromatin landscape (2022) · cites this paper as [57]

    The Results and Discussion show that altering Pknox2 expression in mouse limb-bud mesenchyme perturbs skeletal development, supporting a developmental role for PKNOX2. The paper uses the Prx1 promoter to drive that expression, but the cited passages do not establish a developmental role for PRRX1 itself. No page numbers are available in our copy. (LLM, conf=0.82)

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Research works printed but not linked: 36

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Cited by

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Authors
Zhou, W., Zhu, H., Zhao, J., Li, H., Wan, Y., Cao, J., Zhao, H., Yu, J., Zhou, R., Yao, Y., Zhang, L., Wang, L., He, L., Ma, G., Yao, Z., & Guo, X.
Affiliations
Shanghai Jiao Tong University (CN)
Corresponding author
Guo, Xizhi
ORCID
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2013-05-22
Volume
8
Issue
5
Pages
e64237
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0064237
PMC
PMC3661445
OpenAlex
W1993026678
Cited by
13
References
0
Citations by year
2024: 1 · 2022: 2 · 2021: 2 · 2020: 2 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 2
FWCI
0.45
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China (31171396, 31100624, 2012CB966903, 81121001)
  • National Key Research and Development Program of China (2012CB966903, 31100624, 31171396)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Developmental Biology › Congenital limb and hand anomalies
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
Keywords
limb bud mesenchyme, BMP/Smad signaling, mesenchymal condensation, chondrogenic differentiation, limb development
MeSH terms
Animals, Base Sequence, Mesoderm, Mice, Transgenic, Osteoblasts, Stem Cells, Transcription Factors, DNA Primers, Homeodomain Proteins, Limb Buds, Chondrocytes, Mice

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