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Cited by 29Fungal Biology and ApplicationsDrug-Induced Hepatotoxicity and ProtectionMedicinal plant effects and applications

Hepatoprotective Bioactivity of the Glycoprotein, Antrodan, Isolated from Antrodia cinnamomea Mycelia

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Ker, Yaw-Bee, Peng, Chiung-Chi, Chang, Wan-Lin, Chyau, Charng-Cherng, Peng, Robert Y.·2014·PLoS ONE
DOI 10.1371/journal.pone.0093191PMC PMC3972158
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Abstract

Antrodan, a protein-bound polysaccharide isolated from Antrodia cinnamomea mycelia, was demonstrated to exhibit significant anti-inflammatory bioactivity in vitro. However, its role in hepatic injury in vivo still remains unclear. We hypothesized that antrodan may have beneficial hepatoprotective effects. To verify this, a lipopolysaccharide (LPS)-Sprague-Dawley rat model was used. Antrodan protected against liver damage by suppressing LPS-stimulated serum glutamine-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT), interleukin (IL)-6, hepatic thiobarbituric acid reactive substances (TBARS), nitric oxide (NO), inducible NO synthase (iNOS) and nuclear factor (NF)-κB, and by effectively alleviating the downregulated hepatic superoxide dismutase (SOD), catalase, and glutathione peroxidase (GSH-Px). Hematoxylin-eosin staining revealed that antrodan at a dosage of 40 mg/kg was able to alleviate LPS-induced liver damage to a normal status. In addition, we identified the partial main architectural backbone of antrodan to have a 1 → 3 linear β-glycosidic backbone of mannan linked by β-1 → 3 glucosidic branches. Conclusively, antrodan can potentially ameliorate liver damage in vivo by suppressing oxidative stress induced by LPS.

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  • H&E staining of Day 30 iHLOs revealed epithelial luminal structures.

    “Hematoxylin and eosin (H&E) staining revealed epithelial luminal structures, further substantiating epithelial maturation (Fig. 1G and Supplementary Fig. 1A)27.”

    From ​Temporal proteomic profiling of iPSC-derived human liver organoids reveals optimal maturation for drug metabolism and toxicology (2026) · cites this paper as [27]

    The Materials and Methods section describes H&E staining of liver tissue collected after LPS administration, while the Results section discusses hepatic injury. Our copy does not address Day 30 iHLOs, epithelial luminal structures, or their maturation (searched: iHLOs, epithelial lumen, organoids). (LLM, conf=0.75)

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Authors
Ker, Y.-B., Peng, C.-C., Chang, W.-L., Chyau, C.-C., & Peng, R. Y.
Affiliations
  • Hungkuang University (TW)
  • Taipei Medical University (TW)
Corresponding author
  • Ker, Yaw-Bee
  • Chyau, Charng-Cherng
ORCID
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2014-04-01
Volume
9
Issue
4
Pages
e93191
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0093191
PMC
PMC3972158
OpenAlex
W2037850871
Cited by
29
References
0
Citations by year
2026: 2 · 2025: 2 · 2024: 1 · 2023: 1 · 2022: 3 · 2020: 3 · 2019: 1 · 2018: 6 · 2017: 3 · 2016: 2 · 2015: 4 · 2014: 1
FWCI
2.43
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
National Science Council
Topics
  • Health Sciences › Medicine › Pharmacology › Fungal Biology and Applications
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
  • Health Sciences › Medicine › Complementary and alternative medicine › Medicinal plant effects and applications
Keywords
Antrodia cinnamomea, hepatoprotection, protein-bound polysaccharide, mycelium, lipopolysaccharide-induced liver injury, oxidative stress, anti-inflammatory activity, mannan, β-1,3-glucan, antioxidant enzymes, NF-κB, nitric oxide, interleukin-6
MeSH terms
Animals, Antioxidants, Fungal Proteins, Glycoproteins, Liver, Liver Function Tests, Male, Nitric Oxide, Oxidation-Reduction, Interleukin-6, NF-kappa B, Thiobarbituric Acid Reactive Substances, Protective Agents, Mycelium, Rats, Nitric Oxide Synthase Type II, Antrodia

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