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Cited by 23MicroRNA in disease regulationInvertebrate Immune Response MechanismsPhagocytosis and Immune Regulation

The Involvement of MiR-1-Clathrin Pathway in the Regulation of Phagocytosis

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Liu, Cuilian, Wang, Jiajia, Zhang, Xiaobo·2014·PLoS ONE
DOI 10.1371/journal.pone.0098747PMC PMC4059620
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Abstract

Phagocytosis, one of the most powerful immune responses, is a complicated process regulated by many factors. However the regulation of phagocytosis mediated by microRNAs has not been extensively investigated. To address this issue, the regulation of phagocytosis by miR-1 was characterized in this study. The results showed that miR-1 played an important role in the phagocytosis regulation in shrimp in vivo. The sequence analysis indicated that miR-1 was highly conserved from invertebrates to mammals, suggesting that miR-1 might share the similar or same functions in phagocytosis of shrimp hemocytes and mammalian macrophages. The data presented that miR-1 was significantly downregulated in cancerous macrophage RAW264.7 cells compared with those in the isolated murine macrophage and in the immortalized macrophage ANA-1. The findings showed that miR-1 had a great effect on the regulation of phagocytosis in cancerous macrophage by the inhibition of clathrin heavy chain 1 (CLTC1) gene. Therefore our study presented a novel miR-1-mediated regulation of phagocytosis both in invertebrate and in vertebrate.

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  • Overexpression of miR-1 decreases phagocytic uptake of E. coli by targeting CLTC1.

    “Experimental evaluation confirmed that overexpression of miR-1 decreases phagocytic uptake of E. coli by targeting clathrin heavy chain 1 (CLTC1) [111].”

    From MicroRNAs as Regulators of Phagocytosis (2022) · cites this paper as [111]

    Results §2 reports that miR-1 overexpression significantly decreased uptake of FITC-labeled E. coli in RAW264.7 macrophages, but had no effect in ANA-1 macrophages. Results §3 identifies CLTC1 as a miR-1 target involved in phagocytosis. The claim omits the cell-type limitation on the observed decrease. (LLM, conf=0.88)

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Research works printed but not linked: 29

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Cited by

Sorted by citations

Authors
Liu, C., Wang, J., & Zhang, X.
Affiliations
Zhejiang University (CN)
Corresponding author
Zhang, Xiaobo
ORCID
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2014-06-16
Volume
9
Issue
6
Pages
e98747
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0098747
PMC
PMC4059620
OpenAlex
W2051447533
Cited by
23
References
0
Citations by year
2025: 1 · 2024: 1 · 2022: 2 · 2021: 2 · 2020: 2 · 2019: 3 · 2018: 3 · 2017: 5 · 2016: 2 · 2015: 2
FWCI
0.96
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National High-tech Research and Development Program (2012AA092205-2, 2012AA092205)
  • National Key Research and Development Program of China (2012AA092205, 2012CB114403)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Immunology and Microbiology › Immunology › Invertebrate Immune Response Mechanisms
  • Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
Keywords
miR-1, microRNA, phagocytosis, shrimp hemocytes, macrophages, RAW 264.7 macrophages, innate immunity
MeSH terms
Animals, Base Sequence, Cell Line, Crustacea, Insecta, Macrophages, Phagocytosis, Gene Expression Regulation, Neoplastic, Conserved Sequence, Clathrin Heavy Chains, MicroRNAs, Mice

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