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Cited by 93Fluid Dynamics and MixingPluripotent Stem Cells ResearchFluid Dynamics and Turbulent Flows

Flow Characterization of a Spinner Flask for Induced Pluripotent Stem Cell Culture Application

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Ismadi, Mohd-Zulhilmi, Gupta, Priyanka, Fouras, Andreas, Verma, Paul, Jadhav, Sameer, Bellare, Jayesh, Hourigan, Kerry·2014·PLoS ONE
DOI 10.1371/journal.pone.0106493PMC PMC4184809
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Abstract

We present detailed quantitative measurement analyses for flow in a spinner flask with spinning rates between 20 to 45 RPM, utilizing the optical velocimetry measurement technique of Particle Image Velocimetry (PIV). A partial section of the impeller was immersed in the working fluid to reduce the shear forces induced on the cells cultured on microcarriers. Higher rotational speeds improved the mixing effect in the medium at the expense of a higher shear environment. It was found that the mouse induced pluripotent stem (iPS) cells achieved the optimum number of cells over 7 days in 25 RPM suspension culture. This condition translates to 0.0984 Pa of maximum shear stress caused by the interaction of the fluid flow with the bottom surface. However, inverse cell growth was obtained at 28 RPM culture condition. Such a narrow margin demonstrated that mouse iPS cells cultured on microcarriers are very sensitive to mechanical forces. This study provides insight to biomechanical parameters, specifically the shear stress distribution, for a commercially available spinner flask over a wide range of Reynolds number.

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  • PSCs are sensitive to mechanical cues, and changes in shear stress due to increases in reactor volume, impeller speed, and impeller size will require careful optimization.

    “Critically, PSCs are sensitive to mechanical cues,179,180 and changes in shear stress due to increases in reactor volume as well as impeller speed and size will require careful optimization.”

    From Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes (2023) · cites this paper as [180]

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References 36 · Cited by 93

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Authors
Ismadi, M.-Z., Gupta, P., Fouras, A., Verma, P., Jadhav, S., Bellare, J., & Hourigan, K.
Affiliations
  • Monash University (AU)
  • Indian Institute of Technology Bombay (IN)
  • South Australian Research and Development Institute (AU)
Corresponding author
Ismadi, Mohd-Zulhilmi
ORCID
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2014-10-03
Volume
9
Issue
10
Pages
e106493
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0106493
PMC
PMC4184809
OpenAlex
W1972264416
Cited by
93
References
36
Citations by year
2026: 5 · 2025: 8 · 2024: 6 · 2023: 4 · 2022: 3 · 2021: 9 · 2020: 10 · 2019: 10 · 2018: 14 · 2017: 9 · 2016: 5 · 2015: 9 · 2014: 1
FWCI
4.15
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Monash University Research Portal (Monash University) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • DSpace (IIT Bombay) (submittedVersion)
Funders
Australian Research Council (DP110100434)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Fluid Dynamics and Mixing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Physical Sciences › Engineering › Computational Mechanics › Fluid Dynamics and Turbulent Flows
Keywords
spinner flasks, induced pluripotent stem cells, microcarrier culture, shear stress, cell growth, impeller speed, mixing, cell culture bioreactors, flow characterization, Reynolds number
MeSH terms
Animals, Rheology, Cell Culture Techniques, Bioreactors, Cell Proliferation, Mice, Induced Pluripotent Stem Cells, Batch Cell Culture Techniques

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