Loss of Infectivity of HIV-1 Particles Produced by Mobile Lymphocytes
Open accessAbstract
HIV-1 spreads by cell-free particles and through direct cell contacts. To discriminate between these two modes of dissemination, an assay in which the cells are cultured under shaking conditions impairing cell-to-cell transmission has been described. We addressed the impact of shaking on HIV-1 particle infectivity. Kinetics of HIV-1 infection in static or shaking conditions confirmed that HIV-1 replication is reduced in mobile lymphocyte T cells. Strikingly, the infectivity of viruses produced by mobile lymphocytes was dramatically reduced. In parallel, the amount of envelope protein present on these particles showed a continuous decrease over time. We conclude that inefficient HIV-1 replication in mobile lymphocytes in this experimental system is not only due to avoidance of viral cell-to-cell transfer but also to the loss of infectivity of the viral particles due to the alteration of the composition and functionality of the particles produced by these lymphocytes. It is important to take these observations into account when studying viral transmission under shaking conditions.
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Chazal et al. reported poor efficiency of HIV-1 replication in shaking culture of HIV-1-infected T cells was related to loss of infectivity of cell-free virions produced by shaky lymphocytes.
“However, Chazal et al. reported later that the poor efficiency of HIV-1 replication in the shaking culture of HIV-1-infected T cells was related to the loss of infectivity of cell-free virions produced by shaky lymphocytes [26].”
From HIV-1 and HTLV-1 Transmission Modes: Mechanisms and Importance for Virus Spread (2022) · cites this paper as [26]
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Research works printed but not linked: 12
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HIV-1 and HTLV-1 Transmission Modes: Mechanisms and Importance for Virus Spread
Kalinichenko, S., Komkov, D., & Mazurov, D. (2022) · Viruses · doi:10.3390/v14010152 · Cited by 68 · see citing works
“reported later that the poor efficiency of HIV-1 replication in the shaking culture of HIV-1-infected T cells was related to the loss of infectivity of cell-free virions produced by shaky lymphocytes [26].”
Background
- Authors
- Chazal, M., Nzounza, P., Pique, C., & Ramirez, B. C.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2014-10-08
- Volume
- 9
- Issue
- 10
- Pages
- e109601
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC4190309
- OpenAlex
- W2020357105
- Cited by
- 4
- References
- 0
- Citations by year
- 2026: 1 · 2024: 1 · 2022: 1 · 2020: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Styrelsen för Internationellt Utvecklingssamarbete
- Sidaction
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases (HIV-1)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
- Health Sciences › Medicine › Infectious Diseases › HIV/AIDS Research and Interventions
- Keywords
- HIV-1, viral infectivity, cell-to-cell transmission, T cells, shaking culture, viral replication kinetics, HIV-1 envelope glycoprotein, HIV transmission routes
- MeSH terms
- Cell Line, Cell Membrane, Cell Movement, Humans, T-Lymphocytes, Virion, Virus Replication, HIV-1, Gene Expression Regulation, Viral, Cell Culture Techniques, env Gene Products, Human Immunodeficiency Virus
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