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Cited by 85Cellular Mechanics and InteractionsCell Adhesion Molecules ResearchProstate Cancer Treatment and Research

Differential effects of tissue culture coating substrates on prostate cancer cell adherence, morphology and behavior

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Liberio, Michelle S., Sadowski, Martin C., Soekmadji, Carolina, Davis, Rohan A., Nelson, Colleen C.·2014·PLoS ONE
DOI 10.1371/journal.pone.0112122PMC PMC4223027
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Abstract

Weak cell-surface adhesion of cell lines to tissue culture surfaces is a common problem and presents technical limitations to the design of experiments. To overcome this problem, various surface coating protocols have been developed. However, a comparative and precise real-time measurement of their impact on cell behavior has not been conducted. The prostate cancer cell line LNCaP, derived from a patient lymph node metastasis, is a commonly used model system in prostate cancer research. However, the cells' characteristically weak attachment to the surface of tissue culture vessels and cover slips has impeded their manipulation and analysis and use in high throughput screening. To improve the adherence of LNCaP cells to the culture surface, we compared different coating reagents (poly-L-lysine, poly-L-ornithine, collagen type IV, fibronectin, and laminin) and culturing conditions and analyzed their impact on cell proliferation, adhesion, morphology, mobility and gene expression using real-time technologies. The results showed that fibronectin, poly-L-lysine and poly-L-ornithine improved LNCaP cells adherence and provoked cell morphology alterations, such as increase of nuclear and cellular area. These coating reagents also induced a higher expression of F-actin and reduced cell mobility. In contrast, laminin and collagen type IV did not improve adherence but promoted cell aggregation and affected cell morphology. Cells cultured in the presence of laminin displayed higher mobility than control cells. All the coating conditions significantly affected cell viability; however, they did not affect the expression of androgen receptor-regulated genes. Our comparative findings provide important insight for the selection of the ideal coating reagent and culture conditions for the cancer cell lines with respect to their effect on proliferation rate, attachment, morphology, migration, transcriptional response and cellular cytoskeleton arrangement.

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  • The extracellular matrix has been shown to influence adhesion, proliferation, mobility, and morphology of rather robust cell lines.

    “However, the extracellular matrix has also been shown to influence adhesion, proliferation, mobility, and morphology of rather robust cell lines (Fiegel et al., 2004; García-Parra et al., 2013; Liberio et al., 2014).”

    From In Vitro research Reproducibility: Keeping up High Standards (2019) · cites this paper as Liberio et al., 2014

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Cited by

Sorted by citations

Authors
Liberio, M. S., Sadowski, M. C., Soekmadji, C., Davis, R. A., & Nelson, C. C.
Affiliations
  • Translational Research Institute (AU)
  • Griffith University (AU)
Corresponding author
Nelson, Colleen C.
ORCID
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2014-11-06
Volume
9
Issue
11
Pages
e112122
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0112122
PMC
PMC4223027
OpenAlex
W2015973399
Cited by
85
References
2
Citations by year
2026: 5 · 2025: 2 · 2024: 3 · 2023: 5 · 2022: 8 · 2021: 13 · 2020: 10 · 2019: 10 · 2018: 9 · 2017: 8 · 2016: 7 · 2015: 5
FWCI
3.08
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • QUT ePrints (Queensland University of Technology) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
  • Griffith Research Online (submittedVersion, cc-by)
Funders
  • Australian Prostate Cancer Research
  • Australian Government
  • Griffith University
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Prostate Cancer Treatment and Research
Keywords
prostate cancer, LNCaP cells, cell adhesion, poly-L-lysine, poly-L-ornithine, type IV collagen, fibronectin, laminin, cell morphology, cell migration, F-actin, cell aggregation, cell proliferation, high-throughput screening
MeSH terms
Actins, Androgens, Cell Adhesion, Cell Count, Cell Movement, Cell Survival, Collagen, Fibronectins, Humans, Laminin, Male, Peptides, Polylysine, Prostatic Neoplasms, Tumor Cells, Cultured, Cell Culture Techniques, Hydroxymethylglutaryl-CoA Reductase Inhibitors, Simvastatin

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