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Ocular Fluid As a Replacement for Serum in Cell Cryopreservation Media

Varma, Vivek Phani, Devi, Lalitha, Venna, Naresh Kumar, Murthy, Ch Lakshmi N., Idris, Mohammed M., Goel, Sandeep·2015·PLOS ONE
DOI 10.1371/journal.pone.0131291PMC PMC4489643
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Fig 1 Cell viability and cell recovery in fresh and frozen–thawed cells. (A) Cell viability of fresh and frozen-thawed cells as determined by trypan blue dye exclusion immediately after thawing. (B) Percentage of cell recovery of frozen–thawed cells after 24 h (MEF, C18-4, CHO, HEK, and mES cells) and 72 h (hPBMCs and mBMCs) of culture. (C) Cell viability as determined by trypan blue dye exclusion after 24 h (MEF, C18-4, CHO, HEK, and mES cells) and 72h (hPBMCs and mBMCs) of culture. Data represent mean ± SEM off our trials for each cell type in each group. Bars with different letters are significantly different at P < 0.05.
Fig 1 Cell viability and cell recovery in fresh and frozen–thawed cells. (A) Cell viability of fresh and frozen-thawed cells as determined by trypan blue dye exclusion immediately after thawing. (B) Percentage of cell recovery of frozen–thawed cells after 24 h (MEF, C18-4, CHO, HEK, and mES cells) and 72 h (hPBMCs and mBMCs) of culture. (C) Cell viability as determined by trypan blue dye exclusion after 24 h (MEF, C18-4, CHO, HEK, and mES cells) and 72h (hPBMCs and mBMCs) of culture. Data represent mean ± SEM off our trials for each cell type in each group. Bars with different letters are significantly different at P < 0.05.CC BY · PubMed Central
Fig 2 Expression of proliferation-, pro-apoptosis-, anti-apoptosis-, and early apoptosis-specific proteins in frozen-thawed cells. Expression of cell PCNA, BAX, BCL2, and Annexin V respectively in fresh and frozen–thawed cells after 24 h (MEF, C18-4, CHO, HEK, and mES cells) and 72 h (hPBMCs and mBMCs) of culture. Thirty-microgram aliquots of each cell extract were subjected to SDS-PAGE and western blot analysis. (A) Representative western blotting and densitometry analysis of (B) BAX/BCL2 ratio, (C) PCNA, and (D) Annexin V expression relative to GAPDH. Data represent mean ± SEM off our trials for a cell type in each group. Bars with different letters are significantly different at P < 0.05.
Fig 2 Expression of proliferation-, pro-apoptosis-, anti-apoptosis-, and early apoptosis-specific proteins in frozen-thawed cells. Expression of cell PCNA, BAX, BCL2, and Annexin V respectively in fresh and frozen–thawed cells after 24 h (MEF, C18-4, CHO, HEK, and mES cells) and 72 h (hPBMCs and mBMCs) of culture. Thirty-microgram aliquots of each cell extract were subjected to SDS-PAGE and western blot analysis. (A) Representative western blotting and densitometry analysis of (B) BAX/BCL2 ratio, (C) PCNA, and (D) Annexin V expression relative to GAPDH. Data represent mean ± SEM off our trials for a cell type in each group. Bars with different letters are significantly different at P < 0.05.CC BY · PubMed Central

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Authors
Varma, V. P., Devi, L., Venna, N. K., Murthy, C. L. N., Idris, M. M., & Goel, S.
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2015-07-02
Volume
10
Issue
7
Pages
e0131291
ISSN
1932-6203
DOI
10.1371/journal.pone.0131291
PMC
PMC4489643
Cited by
18
References
0
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (vor)

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Varma, V. P., Devi, L., Venna, N. K., Murthy, C. L. N., Idris, M. M., & Goel, S. (2015). Ocular Fluid As a Replacement for Serum in Cell Cryopreservation Media. PLOS ONE. https://doi.org/10.1371/journal.pone.0131291