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Cited by 20Birth, Development, and HealthAdipokines, Inflammation, and Metabolic DiseasesAdipose Tissue and Metabolism

Effects of prenatal low protein and postnatal high fat diets on visceral adipose tissue macrophage phenotypes and IL-6 expression in Sprague Dawley rat offspring

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Xie, Linglin, Zhang, Ke, Rasmussen, Dane, Wang, Junpeng, Wu, Dayong, Roemmich, James N., Bundy, Amy, Johnson, W. Thomas +1 more·2017·PLOS ONE
DOI 10.1371/journal.pone.0169581PMC PMC5283658
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Abstract

Adipose tissue macrophages (ATM) are implicated in adipose tissue inflammation and obesity-related insulin resistance. Maternal low protein models result in fetal programming of obesity. The study aims to answer whether maternal undernutrition by protein restriction affects the ATM M1 or M2 phenotype under postnatal high fat diet in F1 offspring. Using a rat model of prenatal low protein (LP, 8% protein) diet followed by a postnatal high fat energy diet (HE, 45% fat) or low fat normal energy diet (NE, 10% fat) for 12 weeks, we investigated the effects of these diets on adiposity, programming of the offspring ATM phenotype, and the associated inflammatory response in adipose tissue. Fat mass in newborn and 12-week old LP fed offspring was lower than that of normal protein (20%; NP) fed offspring; however, the adipose tissue growth rate was higher compared to the NP fed offspring. While LP did not affect the number of CD68+ or CD206+ cells in adipose tissue of NE offspring, it attenuated the number of these cells in offspring fed HE. In offspring fed HE, LP offspring had a lower percentage of CD11c+CD206+ ATMs, whose abundancy was correlated with the size of the adipocytes. Noteworthy, similar to HE treatment, LP increased gene expression of IL-6 within ATMs. Two-way ANOVA showed an interaction of prenatal LP and postnatal HE on IL-6 and IL-1β transcription. Overall, both LP and HE diets impact ATM phenotype by affecting the ratio of CD11c+CD206+ ATMs and the expression of IL-6.

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  • Across the species surveyed, changes in offspring adiposity are accompanied by alterations in adipocyte size or number, insulin signaling and metabolic protein levels, including PPARγ, as well as inflammatory markers and leukocytes in the adipose tissue of the offspring born from low protein diet-fed mothers.

    “Across the species surveyed, changes in offspring adiposity are accompanied by alterations in adipocyte size or number, insulin signaling and metabolic protein levels, including PPARγ, as well as inflammatory markers and leukocytes in the adipose tissue of the offspring born from low protein diet-fed mothers [71, 72, 78, 82].”

    From Developmental programming of offspring adipose tissue biology and obesity risk (2021) · cites this paper as [82]

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Cited by

Sorted by citations

Authors
Xie, L., Zhang, K., Rasmussen, D., Wang, J., Wu, D., Roemmich, J. N., Bundy, A., Johnson, W. T., & Claycombe, K.
Affiliations
  • University of North Dakota (US)
  • Tufts University (US)
  • Agricultural Research Service (US)
Corresponding author
  • Xie, Linglin
  • Claycombe, Kate
ORCID
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2017-01-31
Volume
12
Issue
1
Pages
e0169581
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0169581
PMC
PMC5283658
OpenAlex
W2583443376
Cited by
20
References
2
Citations by year
2024: 1 · 2023: 1 · 2022: 3 · 2021: 4 · 2020: 2 · 2019: 2 · 2018: 7
FWCI
3.29
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, public-domain)
  • Europe PMC (PubMed Central) (publishedVersion, public-domain)
  • PubMed Central (publishedVersion, public-domain)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • OakTrust (Texas A&M University Libraries) (submittedVersion)
  • Journal of Nutrition
Funders
  • National Institutes of Health (1R15HL117238)
  • National Center for Research Resources (5P20RR016471-12/8 P20 GM103442-12)
  • American Heart Association (13SDG14650009)
  • USDA Agricultural Research Service (3062-51000-052-00D)
Topics
  • Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Birth, Development, and Health
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
Keywords
maternal protein restriction, fetal programming, macrophage polarization, interleukin-6, IL-1β, high-fat diet, adipose tissue inflammation, adiposity, adipocyte size, insulin resistance, Sprague-Dawley rats
MeSH terms
Animals, Animals, Newborn, Cell Separation, Female, Macrophages, Male, Models, Biological, Organ Size, Phenotype, Pregnancy, Transcription, Genetic, Biomarkers, Antigens, CD, Interleukin-6, Rats, Sprague-Dawley, Diet, Protein-Restricted, Intra-Abdominal Fat, Interleukin-1beta, Diet, High-Fat, Real-Time Polymerase Chain Reaction

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