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Cited by 12Vanadium and Halogenation ChemistryMolecular Biology Techniques and ApplicationsRNA Research and Splicing

Application of ribonucleoside vanadyl complex (RVC) for developing a multifunctional tissue preservative solution

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Shieh, Tzong-Ming, Chen, Chi-Yuan, Hsueh, Chuen, Yu, Cheng-Chia, Chen, Chin-Chuan, Wang, Tong-Hong·2018·PLOS ONE
DOI 10.1371/journal.pone.0194393PMC PMC5851642
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Abstract

The quality of biological samples greatly affects the accuracy of scientific results. However, RNA in cryopreserved tissues gradually degrades during storage, leading to errors in the results of subsequent experiments. A suitable sample preservative solution can prolong storage and enhance the research value of samples. Here, we developed a sample preservative solution using the properties of the ribonucleoside vanadyl complex (RVC) and compared its effects on RNA and DNA quality, protein activity, and tissue morphology with the commercially available and widely used RNAlater® Stabilization Solution. The results showed that both the RVC-based preservative solution and RNAlater can effectively delay RNA degradation in tissue samples stored at 4°C or -80°C compared with samples stored without any preservative solution. In contrast to RNAlater, the RVC-based preservative solution did not result in damage to the tissue morphology or a loss of protein activity. Additionally, the RVC-based preservative solution did not affect the RNA and genomic DNA contents of the tissue samples or the results of subsequent experimental analyses. An RVC-based reagent can be used as a multifunctional yet relatively inexpensive tissue preservative solution to provide a comprehensive and cost-effective method for preserving samples for tissue banks.

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Authors
Shieh, T.-M., Chen, C.-Y., Hsueh, C., Yu, C.-C., Chen, C.-C., & Wang, T.-H.
Affiliations
  • China Medical University (TW)
  • Chang Gung University of Science and Technology (TW)
  • Chang Gung Memorial Hospital (TW)
  • Chung Shan Medical University (TW)
Corresponding author
Wang, Tong-Hong
ORCID
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2018-03-14
Volume
13
Issue
3
Pages
e0194393
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0194393
PMC
PMC5851642
OpenAlex
W2793522306
Cited by
12
References
0
Citations by year
2025: 2 · 2024: 3 · 2023: 3 · 2022: 1 · 2020: 3
FWCI
0.27
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Ministry of Science and Technology, Taiwan (MOST 105-2320-B-182A-004-)
  • Chang Gung Medical Foundation (CRRPG3F0071)
Topics
  • Physical Sciences › Chemistry › Inorganic Chemistry › Vanadium and Halogenation Chemistry
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords
RNA degradation, cryopreservation, tissue banking, RNAlater, DNA integrity, protein activity, tissue morphology, sample preservation
MeSH terms
Animals, Male, Mice, Inbred BALB C, Ribonucleosides, Tissue Preservation, Vanadates, Organ Preservation Solutions, RNA Stability, Mice, Protein Stability

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