Comparison of iron-reduced and iron-supplemented semisynthetic diets in T cell transfer colitis
Open accessAbstract
Clinical observations in inflammatory bowel disease patients and experimental studies in rodents suggest that iron in the intestinal lumen derived from iron-rich food or oral iron supplementation could exacerbate inflammation and that iron depletion from the diet could be protective. To test the hypothesis that dietary iron reduction is protective against colitis development, the impact of iron reduction in the diet below 10 mg/kg on the course of CD4+ CD62L+ T cell transfer colitis was investigated in adult C57BL/6 mice. Weight loss as well as clinical and histological signs of inflammation were comparable between mice pretreated with semisynthetic diets with either < 10mg/kg iron content or supplemented with 180 mg/kg iron in the form of ferrous sulfate or hemin. Accumulation and activation of Ly6Chigh monocytes, changes in dendritic cell subset composition and induction of proinflammatory Th1/Th17 cells in the inflamed colon were not affected by the iron content of the diets. Thus, dietary iron reduction did not protect adult mice against severe intestinal inflammation in T cell transfer induced colitis.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
This results in activation of NF-κB and increased production of proinflammatory cytokines TNF, IL-1 and IL-6.
“This results in activation of one of the central transcription factors of the immune response, NF-κB, and in increased production of proinflammatory cytokines TNF, IL-1 and IL-6 (Buhnik-Rosenblau et al., 2012;Mahalhal et al., 2018;Markota et al., 2019).”
From Iron in infection and immunity (2020) · cites this paper as Markota et al., 2019
The Introduction states that excess luminal iron can activate NF-κB signaling and induce inflammatory cytokine production. It does not identify TNF, IL-1 and IL-6 as the cytokines produced through that pathway, so the named-cytokine portion is not supported by this paper's text. (LLM, conf=0.87)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 50 · Cited by 9
- Authors
- Markota, A., Metzger, R., Heiseke, A. F., Jandl, L., Dursun, E., Eisenächer, K., Reindl, W., Haller, D., & Krug, A. B.
- Affiliations
- Ludwig-Maximilians-Universität München (DE)
- Technical University of Munich (DE)
- Corresponding author
- Krug, Anne B.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2019-07-05
- Volume
- 14
- Issue
- 7
- Pages
- e0218332
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC6611680
- OpenAlex
- W2953875484
- Cited by
- 9
- References
- 50
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 1
- FWCI
- 0.90
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Open access LMU (Ludwid Maxmilian's Universitat Munchen) (submittedVersion, cc-by)
- Figshare (submittedVersion, cc-by)
- mediaTUM (Technical University of Munich) (submittedVersion, other-oa)
- Funders
- Deutsche Forschungsgemeinschaft (GRK 1482, SFB 1054 TP A06, KR2199/3-2, KR2199/9-1, KR2199/6-1)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- iron supplementation, T cell transfer colitis, intestinal inflammation, inflammatory bowel disease, semi-synthetic diet, ferrous sulfate, hemin, Ly6Chi monocytes, dendritic cells, Th1/Th17 cells, CD4+ T cells, C57BL/6 mice
- MeSH terms
- Animals, Colon, Dendritic Cells, Disease Models, Animal, Food, Formulated, Iron, Monocytes, Inflammatory Bowel Diseases, Mice, Knockout, Th1 Cells, Adoptive Transfer, Dietary Supplements, Mice, Th17 Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
