The DOI matches its Crossref record; 66 of the 66 references it cites were found in a registry or an index.
Pseudomonas aeruginosa pili and flagella mediate distinct binding and signaling events at the apical and basolateral surface of airway epithelium.
Open accessAbstract
Pseudomonas aeruginosa, an important opportunistic pathogen of man, exploits numerous factors for initial attachment to the host, an event required to establish bacterial infection. In this paper, we rigorously explore the role of two major bacterial adhesins, type IV pili (Tfp) and flagella, in bacterial adherence to distinct host receptors at the apical (AP) and basolateral (BL) surfaces of polarized lung epithelial cells and induction of subsequent host signaling and pathogenic events. Using an isogenic mutant of P. aeruginosa that lacks flagella or utilizing beads coated with purified Tfp, we establish that Tfp are necessary and sufficient for maximal binding to host N-glycans at the AP surface of polarized epithelium. In contrast, experiments utilizing a P. aeruginosa isogenic mutant that lacks Tfp or using beads coated with purified flagella demonstrate that flagella are necessary and sufficient for maximal binding to heparan sulfate (HS) chains of heparan sulfate proteoglycans (HSPGs) at the BL surface of polarized epithelium. Using two different cell-free systems, we demonstrate that Tfp-coated beads show highest binding affinity to complex N-glycan chains coated onto plastic plates and preferentially aggregate with beads coated with N-glycans, but not with single sugars or HS. In contrast, flagella-coated beads bind to or aggregate preferentially with HS or HSPGs, but demonstrate little binding to N-glycans. We further show that Tfp-mediated binding to host N-glycans results in activation of phosphatidylinositol 3-kinase (PI3K)/Akt pathway and bacterial entry at the AP surface. At the BL surface, flagella-mediated binding to HS activates the epidermal growth factor receptor (EGFR), adaptor protein Shc, and PI3K/Akt, and induces bacterial entry. Remarkably, flagella-coated beads alone can activate EGFR and Shc. Together, this work provides new insights into the intricate interactions between P. aeruginosa and lung epithelium that may be potentially useful in the development of novel treatments for P. aeruginosa infections.
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“For example the exposure of apical receptors like integrins permit the adhesion of bacteria such as S. aureus and P. aeruginosa (Sanford et al., 1978; Davison and Sanford, 1981; Bucior et al., 2012; Smith and McCullers, 2014).”
From Secondary bacterial infections associated with influenza pandemics (2017) · cites this paper as Bucior et al., 2012
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Cites
Principles and Practice of Infectious Diseases.
(1991) · Annals of Internal Medicine · doi:10.7326/0003-4819-114-9-818_5 · Cited by 12,045
Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases
Castagnola, E., Viscoli, C., & Mikulska, M. (2015) · Elsevier eBooks · doi:10.1016/c2012-1-00075-6 · Cited by 2,587
Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients
Smith, E. E., Buckley, D. G., Wu, Z., Saenphimmachak, C., Hoffman, L. R., D’Argenio, D. A., Miller, S. I., Ramsey, B. W., Speert, D. P., Moskowitz, S. M., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0602138103 · Cited by 1,319
Type IV Pili and Twitching Motility
Mattick, J. S. (2002) · Annual Review of Microbiology · doi:10.1146/annurev.micro.56.012302.160938 · Cited by 1,257
Innate immune recognition of bacterial ligands by NAIPs determines inflammasome specificity
Kofoed, E. M., & Vance, R. E. (2011) · Nature · doi:10.1038/nature10394 · Cited by 894
Lightfield, K. L., Persson, J., Brubaker, S. W., Witte, C. E., von Moltke, J., Dunipace, E. A., Henry, T., Sun, Y., Cado, D., Dietrich, W. F., et al. (2008) · Nature Immunology · doi:10.1038/ni.1646 · Cited by 502
Role of Flagella in Pathogenesis ofPseudomonas aeruginosaPulmonary Infection
Feldman, M. F., Bryan, R. A., Rajan, S., Scheffler, L., Brunnert, S. R., Tang, H. B., & Prince, A. S. (1998) · Infection and Immunity · doi:10.1128/iai.66.1.43-51.1998 · Cited by 489
Dasgupta, N., Wolfgang, M. C., Goodman, A. L., Arora, S. K., Jyot, J., Lory, S. J., & Ramphal, R. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03740.x · Cited by 451
Vallet-Gély, I., Olson, J. W., Lory, S. J., Lazdunski, A. M., & Filloux, A. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.111551898 · Cited by 355
Saiman, L. M., & Prince, A. S. (1993) · Journal of Clinical Investigation · doi:10.1172/jci116779 · Cited by 347
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Li, D., Zhao, L., Qu, T., Liu, S., Guo, B., Wang, P., Chen, Y., & Wu, W. (2026) · Sensors and Actuators Reports · doi:10.1016/j.snr.2026.100452
Ringot-Destrez, B. (2018) · theses.fr (ABES) · doi:10.70675/f7ae5fcfz9161z42f4zaafez00d9e3f000ad
Wang, Y., Wang, Y., Zhu, Z., Wang, Q., Liu, X., Cai, Q., Zhang, Y., Li, Y., Zhao, H., Xx, C., et al. (2026) · Human Vaccines & Immunotherapeutics · doi:10.1080/21645515.2026.2689214
“23 Together, three pathways provide a coherent mechanistic framework for the inhibition of PA103 biofilm development by 16G10.”
Intracellular Pseudomonas aeruginosa: internalization and survival
Tejada-Arranz, A., Sunder, V., & Basler, M. (2026) · Trends in Microbiology · doi:10.1016/j.tim.2026.07.004
Boyne, K. L., Broeking, C., Blackstone, M., Singaraju, S., Kelley, T. J., Corey, D. A., & Seed, P. C. (2026) · Infection and Immunity · doi:10.1128/iai.00225-25
“For example, Bucior et al. demonstrated that PA targets distinct epithelial receptors based on cell polarity (17).”
Otterbeck, A., Skorup, P., Larsson, A., Stålberg, J., Baumgarten, M., Lampinen, M., Hjelmqvist, H., & Lipcsey, M. (2026) · Intensive Care Medicine Experimental · doi:10.1186/s40635-026-00952-y
“In the pathogenesis of P. aeruginosa pneumonia, bacteria attach to epithelial surface receptors through adhesins, such as those found in the flagellum and pili of P. aeruginosa [27].”
Calcium promotes Pseudomonas aeruginosa adherence to airway epithelial cells during infection
Zhang, Y., Luthra, D., Lutze, T., Khanam, S., Patrauchan, M. A., & Lutter, E. I. (2026) · Infection and Immunity · doi:10.1128/iai.00430-26
“Several host surface molecules have been described as receptors for P. aeruginosa adhesins, including asialo-GM1, glycosphingolipids (Gb3), N-glycans, integrins, and heparan sulfate proteoglycans (HSPGs) (30, 33, 115–118).”
Tesler, A. B., Goldmann, W. H., & Mazare, A. (2026) · Scientific Reviews · doi:10.1038/s44573-026-00009-x
BindingDB Entry 50007397: Glycocluster Tetrahydroxamic Acids Exhibiting Unprecedented Inhibition of
BindingDB (2021) · UC San Diego · doi:10.7270/q2db8554
Metadata
- Authors
- Bucior, I., Pielage, J. F., & Engel, J. N.
- Affiliations
- University of California, San Francisco (US)
- Corresponding author
- Bucior, Iwona
- Pielage, Julia F.
- Engel, Joanne N.
- ORCID
- Engel, Joanne N.
- Venue
- PLoS Pathogens
- Publisher
- Public Library of Science (PLoS)
- Published
- 2012-04-05
- Volume
- 8
- Issue
- 4
- Pages
- e1002616
- ISSN
- 1553-7366 (ISSN-L)
- 1553-7374
- PMC
- PMC3320588
- OpenAlex
- W2073200952
- Cited by
- 221
- References
- 66
- Citations by year
- 2026: 9 · 2025: 17 · 2024: 13 · 2023: 21 · 2022: 15 · 2021: 13 · 2020: 17 · 2019: 17 · 2018: 15 · 2017: 20 · 2016: 14 · 2015: 10 · 2014: 24 · 2013: 14 · 2012: 2
- FWCI
- 6.42
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS Pathogens (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Funders
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases (P01AI053194, R01AI065902)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Keywords
- Pseudomonas aeruginosa, type IV pili, bacterial flagellum, bacterial adhesion, lung epithelium, N-glycans, heparan sulfate proteoglycans, phosphatidylinositol 3-kinase/Akt signaling, epidermal growth factor receptor, Shc adaptor protein, bacterial invasion, host-pathogen interaction, airway infection
- MeSH terms
- Bacterial Adhesion, Cell Line, Enzyme Activation, Flagella, Heparin, Humans, Lung, Mutation, Fimbriae, Bacterial, Pseudomonas aeruginosa, Pseudomonas Infections, Adhesins, Bacterial, Heparan Sulfate Proteoglycans, Phosphatidylinositol 3-Kinases, Respiratory Mucosa, Proto-Oncogene Proteins c-akt, ErbB Receptors
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