The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Compatibility in the Ustilago maydis–Maize Interaction Requires Inhibition of Host Cysteine Proteases by the Fungal Effector Pit2
Open accessAbstract
The basidiomycete Ustilago maydis causes smut disease in maize, with large plant tumors being formed as the most prominent disease symptoms. During all steps of infection, U. maydis depends on a biotrophic interaction, which requires an efficient suppression of plant immunity. In a previous study, we identified the secreted effector protein Pit2, which is essential for maintenance of biotrophy and induction of tumors. Deletion mutants for pit2 successfully penetrate host cells but elicit various defense responses, which stops further fungal proliferation. We now show that Pit2 functions as an inhibitor of a set of apoplastic maize cysteine proteases, whose activity is directly linked with salicylic-acid-associated plant defenses. Consequently, protease inhibition by Pit2 is required for U. maydis virulence. Sequence comparisons with Pit2 orthologs from related smut fungi identified a conserved sequence motif. Mutation of this sequence caused loss of Pit2 function. Consequently, expression of the mutated protein in U. maydis could not restore virulence of the pit2 deletion mutant, indicating that the protease inhibition by Pit2 is essential for fungal virulence. Moreover, synthetic peptides of the conserved sequence motif showed full activity as protease inhibitor, which identifies this domain as a new, minimal protease inhibitor domain in plant-pathogenic fungi.
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Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans
Haas, B. J., Kamoun, S., Zody, M. C., Jiang, R. H. Y., Handsaker, R. E., Cano, L. M., Grabherr, M., Kodira, C. D., Raffaele, S., Torto-Alalibo, T., et al. (2009) · Nature · doi:10.1038/nature08358 · Cited by 1,471
Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis
Kämper, J., Kahmann, R., Bölker, M., Ma, L.-J., Brefort, T., Saville, B. J., Banuett, F., Kronstad, J. W., Gold, S. E., Müller, O., et al. (2006) · Nature · doi:10.1038/nature05248 · Cited by 1,208
Storage of competent cells for Agrobacterium transformation
Hofgen, R., & Willmitzer, L. (1988) · Nucleic Acids Research · doi:10.1093/nar/16.20.9877 · Cited by 1,037
Barrett, A. J., Kembhavi, A. A., Brown, M. A., Kirschke, H., Knight, C. G., Tamai, M., & Hanada, K. (1982) · Biochemical Journal · doi:10.1042/bj2010189 · Cited by 1,026
Stergiopoulos, I., & de Wit, P. J. G. M. (2009) · Annual Review of Phytopathology · doi:10.1146/annurev.phyto.112408.132637 · Cited by 899
Metabolic priming by a secreted fungal effector
Djamei, A., Schipper, K., Rabe, F., Ghosh, A., Vincon, V., Kahnt, J., Osorio, S., Tohge, T., Fernie, A. R., Feussner, I., et al. (2011) · Nature · doi:10.1038/nature10454 · Cited by 616
Regulation and secretion of Xanthomonas virulence factors
Büttner, D., & Bonas, U. (2010) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2009.00192.x · Cited by 551
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Metadata
- Authors
- Mueller, A. N., Ziemann, S., Treitschke, S., Aßmann, D., & Doehlemann, G.
- Venue
- PLoS Pathogens
- Publisher
- Public Library of Science (PLoS)
- Published
- 2013-02-14
- Volume
- 9
- Issue
- 2
- Pages
- e1003177
- ISSN
- 1553-7366 (ISSN-L)
- 1553-7374
- OpenAlex
- W2001128892
- Cited by
- 275
- References
- 41
- FWCI
- 23.94
- Type
- journal article
- Language
- en
- Open access
- gold
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- Mueller, A. N., Ziemann, S., Treitschke, S., Aßmann, D., & Doehlemann, G. (2013). Compatibility in the Ustilago maydis–Maize Interaction Requires Inhibition of Host Cysteine Proteases by the Fungal Effector Pit2. PLoS Pathogens. https://doi.org/10.1371/journal.ppat.1003177
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