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Cited by 67MicroRNA in disease regulationImmune Response and InflammationMicrobial infections and disease research

Antagonism of miR-328 Increases the Antimicrobial Function of Macrophages and Neutrophils and Rapid Clearance of Non-typeable Haemophilus Influenzae (NTHi) from Infected Lung

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Tay, Hock L., Kaiko, Gerard E., Plank, Maximilian, Li, JingJing, Maltby, Steven, Essilfie, Ama-Tawiah, Jarnicki, Andrew, Yang, Ming +3 more·2015·PLOS Pathogens
DOI 10.1371/journal.ppat.1004549PMC PMC4404141
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Abstract

Pathogenic bacterial infections of the lung are life threatening and underpin chronic lung diseases. Current treatments are often ineffective potentially due to increasing antibiotic resistance and impairment of innate immunity by disease processes and steroid therapy. Manipulation miRNA directly regulating anti-microbial machinery of the innate immune system may boost host defence responses. Here we demonstrate that miR-328 is a key element of the host response to pulmonary infection with non-typeable haemophilus influenzae and pharmacological inhibition in mouse and human macrophages augments phagocytosis, the production of reactive oxygen species, and microbicidal activity. Moreover, inhibition of miR-328 in respiratory models of infection, steroid-induced immunosuppression, and smoke-induced emphysema enhances bacterial clearance. Thus, miRNA pathways can be targeted in the lung to enhance host defence against a clinically relevant microbial infection and offer a potential new anti-microbial approach for the treatment of respiratory diseases.

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  • miR-328 augments phagocytosis and ROS production in Haemophilus influenzae infection.

    “miR-328 is a key element of the host response to Haemophilus influenzae infection as it augments phagocytosis and production of ROS [173].”

    From MicroRNAs as Regulators of Phagocytosis (2022) · cites this paper as [173]

    The Results report that inhibiting miR-328 increased NTHi phagocytosis and ROS production—the opposite of the claim that miR-328 itself augments them. One sentence in the Discussion says miR-328 acts 'by augmenting' these functions, but it conflicts with the reported experiments and the Discussion’s account of inhibition enhancing bacterial clearance. The indexed copy provides section labels but no page numbers. (LLM, conf=0.84)

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Authors
Tay, H. L., Kaiko, G. E., Plank, M., Li, J., Maltby, S., Essilfie, A.-T., Jarnicki, A., Yang, M., Mattes, J., Hansbro, P. M., & Foster, P. S.
Affiliations
Hunter Medical Research Institute (AU)
Corresponding author
  • Yang, Ming
  • Foster, Paul S.
ORCID
Venue
PLOS Pathogens
Publisher
Public Library of Science (PLoS)
Published
2015-04-20
Volume
11
Issue
4
Pages
e1004549
ISSN
  • 1553-7366 (ISSN-L)
  • 1553-7374
DOI
10.1371/journal.ppat.1004549
PMC
PMC4404141
OpenAlex
W2019496443
Cited by
67
References
1
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FWCI
3.53
Type
journal article
Language
en
Update notices
correction (2015-06-24)
Open access
Licence
Locations
  • PLoS Pathogens (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
  • Figshare (submittedVersion)
  • Minerva Access (University of Melbourne) (submittedVersion, cc-by)
  • UTS ePRESS (University of Technology Sydney) (submittedVersion)
  • NOVA (University of Newcastle Australia) (submittedVersion)
Funders
Medical Research Council
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
Keywords
miR-328, nontypeable Haemophilus influenzae, pulmonary infection, innate immunity, macrophages, neutrophils, phagocytosis, reactive oxygen species, microbicidal activity, bacterial clearance, steroid-induced immunosuppression, host defense
MeSH terms
Animals, Flow Cytometry, Fluorescent Antibody Technique, Haemophilus Infections, Haemophilus influenzae, Humans, Macrophages, Male, Mice, Inbred BALB C, Neutrophils, Respiratory Tract Infections, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, MicroRNAs, Mice

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