The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Circulating Methylated SEPT9 DNA in Plasma Is a Biomarker for Colorectal Cancer
Abstract
Background: The presence of aberrantly methylated SEPT9 DNA in plasma is highly correlated with the occurrence of colorectal cancer. We report the development of a new SEPT9 biomarker assay and its validation in case–control studies. The development of such a minimally invasive blood-based test may help to reduce the current gap in screening coverage. Methods: A new SEPT9 DNA methylation assay was developed for plasma. The assay comprised plasma DNA extraction, bisulfite conversion of DNA, purification of bisulfite-converted DNA, quantification of converted DNA by real-time PCR, and measurement of SEPT9 methylation by real-time PCR. Performance of the SEPT9 assay was established in a study of 97 cases with verified colorectal cancer and 172 healthy controls as verified by colonoscopy. Performance based on predetermined algorithms was validated in an independent blinded study with 90 cases and 155 controls. Results: The SEPT9 assay workflow yielded 1.9 μg/L (CI 1.3–3.0) circulating plasma DNA following bisulfite conversion, a recovery of 45%–50% of genomic DNA, similar to yields in previous studies. The SEPT9 assay successfully identified 72% of cancers at a specificity of 93% in the training study and 68% of cancers at a specificity of 89% in the testing study. Conclusions: Circulating methylated SEPT9 DNA, as measured in the new mSEPT9 assay, is a valuable biomarker for minimally invasive detection of colorectal cancer. The new assay is amenable to automation and standardized use in the clinical laboratory.
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Screening and Surveillance for the Early Detection of Colorectal Cancer and Adenomatous Polyps
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Quantification on the LightCycler
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Cited by
Epigenetics of colorectal cancer: biomarker and therapeutic potential
Jung, G., Hernández-Illán, E., Moreira, L., Balaguer, F., & Goel, A. (2020) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/s41575-019-0230-y · Cited by 813
Epigenetic Alterations in Colorectal Cancer: Emerging Biomarkers
Okugawa, Y., Grady, W. M., & Goel, A. (2015) · Gastroenterology · doi:10.1053/j.gastro.2015.07.011 · Cited by 782
Cell-free DNA in the blood as a solid tumor biomarker–a critical appraisal of the literature
Jung, K., Fleischhacker, M., & Rabien, A. (2010) · Clinica Chimica Acta · doi:10.1016/j.cca.2010.07.032 · Cited by 337
Gorgannezhad, L., Umer, M., Islam, M. N., Nguyen, N.-T., & Shiddiky, M. J. A. (2018) · Lab on a Chip · doi:10.1039/c8lc00100f · Cited by 312
A Pan-cancer Transcriptome Analysis Reveals Pervasive Regulation through Alternative Promoters
Demircioğlu, D., Cukuroglu, E., Kindermans, M., Nandi, T., Calabrese, C., Fonseca, N. A., Kahles, A., Lehmann, K.-V., Stegle, O., Brazma, A., et al. (2019) · Cell · doi:10.1016/j.cell.2019.08.018 · Cited by 250
Metadata
- Authors
- deVos, T., Tetzner, R., Model, F., Weiss, G., Schuster, M., Distler, J., Steiger, K. V., Grützmann, R., Pilarsky, C., Habermann, J. K., Fleshner, P. R., Oubre, B. M., Day, R., Sledziewski, A. Z., & Lofton-Day, C.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-07-01
- Volume
- 55
- Issue
- 7
- Pages
- 1337-1346
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2142191516
- Cited by
- 522
- References
- 28
- FWCI
- 8.26
- Type
- journal article
- Language
- en
- Open access
- closed
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- deVos, T., Tetzner, R., Model, F., Weiss, G., Schuster, M., Distler, J., Steiger, K. V., Grützmann, R., Pilarsky, C., Habermann, J. K., Fleshner, P. R., Oubre, B. M., Day, R., Sledziewski, A. Z., & Lofton-Day, C. (2009). Circulating Methylated SEPT9 DNA in Plasma Is a Biomarker for Colorectal Cancer. Clinical Chemistry. https://doi.org/10.1373/clinchem.2008.115808
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