The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Expressed Sequence Tags: Medium-Throughput Protocols
Abstract
Generating expressed sequence tags is a simple, cheap, and efficient way to sample the genome of a target organism. An expressed sequence tag (EST) is a single-pass sequence derived from a single complementary DNA (cDNA) clone, and the sequence serves to identify the gene from which it derives. We present a set of tested laboratory protocols for setting up and performing an EST analysis of any chosen species. These medium-throughput protocols do not require dedicated genomics equipment, such as robots, and focus on the use of microtiter plates and multichannels. Using these protocols, a single competent research worker should be able to generate 2000 ESTs in 1 mo. In a nonnormalized library, these 2000 ESTs should identify between 1000 and 1500 different genes, and thus possibly between 10 and 20% of the genes of any target parasite.
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Cites
Base-Calling of Automated Sequencer Traces Using Phred. II. Error Probabilities
Ewing, B., & Green, P. (1998) · Genome Research · doi:10.1101/gr.8.3.186 · Cited by 5,439
CAP3: A DNA Sequence Assembly Program
Huang, X., & Madan, A. (1999) · Genome Research · doi:10.1101/gr.9.9.868 · Cited by 5,261
Cited by
Lévesque, C. A., Brouwer, H., Cano, L., Hamilton, J. P., Holt, C., Huitema, E., Raffaele, S., Robideau, G. P., Thines, M., Win, J., et al. (2010) · Genome Biology · doi:10.1186/gb-2010-11-7-r73 · Cited by 417
TiGER: A database for tissue-specific gene expression and regulation
Liu, X., Yu, X., Zack, D. J., Zhu, H., & Qian, J. (2008) · BMC Bioinformatics · doi:10.1186/1471-2105-9-271 · Cited by 391
Oliver, M. J. (2005) · Integrative and Comparative Biology · doi:10.1093/icb/45.5.788 · Cited by 373
Brandenberger, R., Wei, H., Zhang, S., Lei, S., Murage, J., Fisk, G. J., Li, Y., Xu, C., Fang, R., Guegler, K., et al. (2004) · Nature Biotechnology · doi:10.1038/nbt971 · Cited by 329
A Family of Human MicroRNA Genes from Miniature Inverted-Repeat Transposable Elements
Piriyapongsa, J., & Jordan, I. K. (2007) · PLoS ONE · doi:10.1371/journal.pone.0000203 · Cited by 283
Metadata
- Authors
- Whitton, C., Daub, J., Thompson, M., & Blaxter, M.
- Venue
- Parasite Genomics Protocols
- Publisher
- Humana Press
- Published
- 2004-05-20
- Volume
- 270
- Pages
- 075-092
- OpenAlex
- W2782267736
- Cited by
- 385
- References
- 2
- FWCI
- 58.49
- Type
- book chapter
- Language
- en
- Open access
- closed
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- Whitton, C., Daub, J., Thompson, M., & Blaxter, M. (2004). Expressed Sequence Tags: Medium-Throughput Protocols. Parasite Genomics Protocols. https://doi.org/10.1385/1-59259-793-9%3A075
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